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Research Article

Bioconjugation as a smart immobilization approach for α-amylase enzyme using stimuli-responsive Eudragit-L100 polymer: a robust biocatalyst for applications in pharmaceutical industry

, , , , , & show all
Pages 2361-2368 | Received 07 May 2019, Accepted 28 May 2019, Published online: 13 Jun 2019

Figures & data

Figure 1. Effect of polymer concentration on immobilization yield (%). Amylolytic activity was assayed under standard assay conditions.

Figure 1. Effect of polymer concentration on immobilization yield (%). Amylolytic activity was assayed under standard assay conditions.

Figure 2. Effect of enzyme concentration on immobilization yield (%). Amylolytic activity was assayed under standard assay conditions.

Figure 2. Effect of enzyme concentration on immobilization yield (%). Amylolytic activity was assayed under standard assay conditions.

Figure 3. Effect of pH on amylolytic activity of free and immobilized α-amylase (E-AC).

Figure 3. Effect of pH on amylolytic activity of free and immobilized α-amylase (E-AC).

Figure 4. Effect of temperature on amylolytic activity of free and immobilized α-amylase (E-AC) at various temperatures from 20–80 °C.

Figure 4. Effect of temperature on amylolytic activity of free and immobilized α-amylase (E-AC) at various temperatures from 20–80 °C.

Table 1. Kinetic parameters (Km, Vmax) and amylolytic efficiency of free and bio-conjugated α-amylase enzyme (E-AC).

Figure 5. Reusability of E-AC in repeated amylolytic reaction. Residual activity of the immobilized α-amylase (in %) after six cycles of repeated use (initial activity was taken as 100%).

Figure 5. Reusability of E-AC in repeated amylolytic reaction. Residual activity of the immobilized α-amylase (in %) after six cycles of repeated use (initial activity was taken as 100%).

Table 2. Half-life times (t1/2) for the free and immobilized (E-CA) α-amylase at different temperatures.

Figure 6. Storage stability of free and immobilized α-amylase (E-AC). Residual amylolytic activity was determined at different time intervals under standard assay conditions upon storage at 5 and 25 °C.

Figure 6. Storage stability of free and immobilized α-amylase (E-AC). Residual amylolytic activity was determined at different time intervals under standard assay conditions upon storage at 5 and 25 °C.