1,567
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

Tenderness and flavor of leg cuts from meat goats influenced by calcium chloride injection

, &
Pages 357-363 | Received 28 Jul 2017, Accepted 05 Dec 2017, Published online: 20 Apr 2018

ABSTRACT

This study was conducted to assess the potential of improving tenderness of chevon using calcium chloride (CaCl2) injection and its effect on the palatability characteristics of chevon. Primal leg cuts from meat goats were allotted to one of four treatments: either no injection (control) or injection with water, CaCl2 (food grade, 2.2% w/v), or CaCl2 plus a spice mix. The CaCl2 injection improved tenderness of goat leg cuts, proven by Warner–Bratzler shear force values and sensory panels. Furthermore, panelists were not able to detect off-flavor problems associated with CaCl2 injection. When CaCl2 was injected into goat leg cuts with the beef spice mixture, it resulted in a more desirable flavor. Calcium injection did not influence flavor volatile compounds in cooked chevon leg cuts. The results indicate that CaCl2 plus spice mix injection can be applied to improve tenderness of goat meat without detrimental effects on other sensory characteristics.

Introduction

Health concerns about the negative effects of high-fat diets in humans have increased the development of low-fat products in recent years.[Citation1] Since goat meat (chevon) has a relatively low fat content (3.5%) compared to other red meats, it is an excellent source for preparing low-fat meat entrees.[Citation2,Citation3] Goat carcasses have low intramuscular fat with high levels of linoleic acid (C18:2n6) compared to lamb or beef.[Citation4,Citation5] Despite these nutritional advantages, chevon is not widely consumed by mainstream Americans since it is considered to be lower in palatability than beef or lamb.[Citation6,Citation7]

Meat tenderness may be considered as the most important eating quality attribute that determines consumer acceptability.[Citation8,Citation9] Toughness in meat develops shortly after slaughter (0–24 h), primarily due to sacromere shortening during the process of rigor mortis.[Citation10] Subsequently, meat tenderizes if stored for prolonged periods at refrigerated temperatures. Kannan et al.[Citation11] reported that tenderness of chevon improved moderately by aging; however, the mechanical strength of intramuscular connective tissue remained unchanged even after 12 days of aging. There is overwhelming evidence that the calpain proteolytic system is responsible for postmorterm tenderization of beef and lamb.[Citation12,Citation13] The calcium chloride (CaCl2) application was developed to enhance tenderization by stimulating proteolysis of muscle proteins by calpain enzymes.[Citation14] It has been demonstrated that infusion of carcasses or injecting cuts with CaCl2 improves meat tenderness of young animals.[Citation15,Citation16] The original procedure suggested for tenderizing meat with CaCl2 was to infuse carcasses with 10% (wt/wt) of 0.3 M CaCl2 solution. Since then numerous modifications have been proposed to the original procedure for reduced off-flavor and other palatability side effects.[Citation14,Citation17] Injection of 0.2 M CaCl2 solution at 5% (wt/wt) improved tenderness in retail cuts from lambs and beef cattle without affecting other palatability characteristics.[Citation18,Citation19] However, the extent to which CaCl2 injection can improve tenderness of chevon is unknown and needs to be determined. The objectives of this research were to assess the potential of improving tenderness of chevon using CaCl2 injection and to evaluate the effect of CaCl2 injection on the palatability characteristics of chevon.

Materials and methods

Intact male goats of Kiko × Spanish (n = 16) were raised on pasture with a grain supplement and harvested at approximately 8 months of age. After a 24-h chill period, each carcass was fabricated according to the procedures descried by Olson et al.[Citation20] Barbecue style was selected because carcasses were within the weight range of 9.0–13.5 kg. Primal leg cuts (n = 32) were collected from each carcass. Thirty-two leg cuts were randomly assigned to one of four injection treatments (n = 8 cuts/treatment): control (no injection), water injection, CaCl2 (food grade, 2.2% w/v; Liquidow, The DOW Chemical Co., Midland, MI, USA) injection, or CaCl2 plus spice mix (beef roast seasoning; A.C. Legg, Inc., Calera, AL, USA) injection. The injection was made by a multi-needle injector (Smart Tech model MN-10, Metalbud CO., Podlas 3, Poland) at a pump level to achieve a 5% (wt/wt) increase in weight. Immediately after injection, each leg was cut into 2.5-cm thick using a band saw with a bone-in blade, vacuum-packed (Vacuum Packaging Machine, Koch Supplies Inc., Kansas City, MO, USA) in a single-barrier plastic bag (Cryovac Inc., Duncan, SC, USA), and chilled at 2°C for 4 days. The chilled leg cuts were then stored at −28°C until analyzed.

The Warner–Bratzler shear force (WBSF) values and cooking losses were determined according to the method of Lee et al.[Citation21] Vacuum-packed frozen leg cuts were thawed at 4°C. Thawed leg cuts were cooked in a convection oven (Maytag Corporation, Model MER6550B, Newton, IA, USA) to the internal temperature of 71°C. After cooking, cooked leg cuts were weighed and wrapped in aluminum foil and cooled at 4°C overnight before core removal. The cuts were allowed to come to room temperature by removing them from the refrigerator and placing them on a laboratory countertop for 2 h, and then 1-cm-diameter cores were removed parallel to muscle fiber orientation.[Citation11] Cores were taken from Semimembranosus muscle from individual cuts and WBSF values assessed using a TA-XT2 texture analyzer fitted with a Warner–Bratzler shear attachment (Texture Technologies Corp., Scarsdale, NY, USA). The instrument was set with a 25-kg load cell and a cross-head speed of 200 mm/min. The difference in weight of samples before and after cooking was expressed as a percentage cooking loss.

Leg cuts for sensory evaluation were also cooked in the same manner as those used to measure WBSF, which were cut into 1-cm3 cubes and served warm to the panel. Panelists received two cubes per sample. An eight-member experienced sensory panel evaluated four samples per session (two sessions daily) for tenderness, juiciness, and overall flavor using 9-point scales (9 = extremely tender, juicy, and like flavor; 1 = extremely tough, dry, and dislike flavor).[Citation22]

The flavor volatiles of cooked leg cuts were extracted using a solid phase microextraction (SPME) method and analyzed using gas chromatography (GC).[Citation23] One leg from each leg was cooked as described above. Each cooked cut was immersed into liquid nitrogen and homogenized with a Waring blender (Fisher Scientific, Pittsburgh, PA, USA). Five grams of the homogenized sample was transferred into a 20-mL vial. Each vial was sealed with a PTFE silicon septum (Supelco, Inc., Bellefonte, PA, USA). The vial was heated at 45°C on an SPME sampling stand, which fitted compactly on a Corning heat/stir plate (Model PC-400; Corning Inc., Corning, NY, USA). Subsequently, the volatiles in the headspace were collected for 15 min on a 50/30-mm divinylbenzene/carboxen/polydimethylsiloxane fiber (Supelco) inserted through the silicon septum. The volatiles were desorbed for 5 min by inserting the SPME needle and exposing the fiber directly into the injection port (220°C) of a TRACE GC Ultra (Thermo Electron Corp., Austin, TX, USA), separated on a Supelcowax column (60 m × 0.32 mm i.d.; Supelco), and detected in a flame ionization detector. Helium was used as a carrier gas with a flow rate of 1.6 mL/min. The injection port was in the splitless mode and the column temperature was programmed from 40°C to 230°C at a rate of 4°C/min and holding at 230°C for 10 min.

Volatile compounds were identified using GC-mass spectrometry (MS). Mass spectra were generated by a Thermo Electron GC (TRACE GC Ultra) interfaced to a mass spectrometer (Finnigan TRACE DSQ MS; Thermo Electron Corp.), operated in the electron impact mode with an electron energy of 70 eV, a multiplier voltage of 1100 V, and data collection rate of 1.5 scan/s over a range of m/z 40–450. Volatile compounds were tentatively identified by comparing their mass spectra with those contained in a mass spectra library (Thermo Electron Corp.). All data were analyzed as a completely randomized design using the PROC MIXED procedure of SAS (SAS Inst, Inc., Cary, NC, USA) with goat considered to be a random effect and injection treatment considered to be a fixed effect. Least-squares means were generated and separated statistically separated by pairwise t-test (PDIFF option) protected by the ANOVA F test (P ≤ 0.05).

Results and discussion

Cooking losses and WBSF values of goat leg cuts from different injection treatments are presented in . The percentage loss from cooking was higher (P < 0.05) in the leg cuts injected with CaCl2 plus a spice mix than in uninjected cuts (control). However, no differences (P > 0.05) were found in cooking losses in leg cuts from control and H2O- or CaCl2-injected groups. Cooking loss traits in the present study are not in agreement with the results of Pringle et al.[Citation16] and Wheeler et al.[Citation18] They reported greater cooking loss with calcium injected beef, whereas Koohmaraie et al.[Citation14] reported similar cooking loss with calcium-injected lamb. This inconsistency may be attributed to the species and maturity difference in animals used in these studies, as well as using different retail cuts (muscles). Differences in the percentage loss from cooking were not detected in the cuts among three different injection groups (H2O, CaCl2, and CaCl2 plus spice mix). Increased percentage cooking loss was expected in leg cuts from the injected groups compared with uninjected group because of the added water through the injection. Similar findings have been reported by Wheeler et al.[Citation24] when the same level of CaCl2 was injected into sub-primal cuts of crossbred heifers after 2 days postmortem. However, other researchers have reported that CaCl2 injection of sub-primal cuts from other breeds of beef cattle did not increase their cooking losses.[Citation16,Citation17] The reason for this discrepancy is not known. All three injected treatments (H2O, CaCl2, and CaCl2 plus a spice mix) caused significant reduction in WBSF values of leg cuts from meat goats in the present study. The cuts from CaCl2- or CaCl2 with spice mix-injected groups had lower (P < 0.05) WBSF values than those from H2O-injected or uninjected groups; however, the WBSF values were not different (P > 0.05) between CaCl2 and CaCl2 plus spice mix-injected groups. These results are consistent with previous findings in beef cattle and lambs.[Citation10,Citation16,Citation24] Although the H2O injection improved tenderness of leg cuts from goats, the cuts from H2O-injected groups were tougher than either of the calcium-injected groups. Improving meat tenderness of H2O-injected cuts might be due to the penetration of needles into the cuts when H2O was applied through a multi-needle injector. Calcium-injected cuts improved in meat tenderness due to increased activity of the calpain proteinase systems.[Citation16,Citation17]

Table 1. Injection treatment effects on cooking loss, Warner–Bratzler shear force (WBSF), and sensory attributes of leg cuts.

Sensory evaluation scores of the treated goat leg cuts by an experienced panel are also presented in . Based on the finding of WBSF values, tenderness scores of calcium-injected goat leg cuts from experienced sensory panel were expected to be higher (P < 0.05) than those from control or water-injected groups. As reported by other researchers, tenderness and juiciness scores of sub-primal beef cuts improved due to the calcium injection.[Citation24] However, outcomes of sensory evaluation are not always consistent with the findings of physicochemical properties.[Citation16,Citation17] The cuts from CaCl2 plus spice mix-injected groups had higher (P < 0.05) tenderness and juiciness scores than those from uninjected groups (control). Those from H2O- or CaCl2-injected groups had intermediate scores and were not different (P > 0.05) from either control or CaCl2 plus spice mix-injected groups. No significant differences were found in tenderness and juiciness scores in the cuts among the three injection groups (H2O, CaCl2, and CaCl2 plus spice mix). Injection of CaCl2 plus spice mix increased sensory scores for overall flavor compared to any of the other treatments; however, differences were not detected in the cuts among control, H2O- and CaCl2-injected groups. This indicated that panelists did not find off-flavor problems associated with CaCl2 injection such as metallic, bitter, or livery taste. Wheeler et al.[Citation18] reported similar results for sensory tenderness and off-flavor scores of calcium-injected (5% (wt/wt) of 0.2 M CaCl2 solution) sub-primal cuts of crossbred steers. However, Morgan et al.[Citation17] reported that the 10% (wt/wt) of 0.3 M CaCl2 injection of sub-primal cuts from mature cows resulted in enhanced sensory tenderness, but also in bitterness and metallic flavors. The reason for this off-flavor problem might be due to the higher concentration and/or injection volume of CaCl2 used.

Twenty-seven volatile compounds were isolated and identified from cooked goat leg cuts (). The compounds were grouped based on their chemical functional groups such as aldehydes, hydrocarbons (alkanes and alkenes), ketones, and others. The meaty flavor of red meat develops during cooking through degradation and reactions of water-soluble compounds.[Citation25] Meat lipids also act as a solvent for the volatile compounds that accumulate during cooking of meat. Among the volatile compounds, carbonyl compounds such as aldehydes and ketones are mainly responsible for oxidized flavor deriving from lipid oxidation; however, less responsibility is ascribed to hydrocarbons (alkanes, alkenes, and alkylfurans) and alcohols.[Citation26] In the present study, nine aldehydes were presented in the cooked leg cuts, which consisted of 5-alkanals, 1-alkenal, 2-alkadienals, and benzaldehyde. Most of these compounds are derived from the oxidation of C18 and C20 unsaturated fatty acids.[Citation27] Of the aldehyde groups, hexanal and nonanal were the most relevant aldehydes presented in the cooked leg cuts. Lamikanra and Dupuy[Citation28] reported that pentenal, hexanal, heptanal, 2,3-octanedione, and nonanal were identified as warmed over flavor markers in cooked goat meat. Furthermore, hexanal was considered a principal marker of cooked goat meat warmed over flavor development. No differences (P > 0.05) were found in any of the aldehyde compounds present in the cooked leg cuts in the current study, except hexanal. Only the percentage of hexanal was lower (P < 0.05) in the cuts injected with H2O than in control groups (no injection), yet there was no difference in the concentration of hexanal in the cooked cuts from the two calcium injected groups and H2O-injected or control groups. Volatile aldehyde compounds such as hexanal, heptanal, octanal, nonanal, 2-octenal, 2-decanal, 2,4-nonadienal, and 2,4 decadienal are derived from the oxidation of C18 unsaturated fatty acids.[Citation26,Citation29] From the degradation of either oleic or linoleic acid, heptanal, octanal, and nonanal are produced. Hexanal, 2-octenal, 2,4 decadienal, and 2,4-nonadienal are generated by the oxidation of linoleic acid, whereas decanal is formed by the oxidation of oleic acid.[Citation26,Citation29]

Table 2. Injection treatment effects on volatile flavors of cooked leg cuts.

Of eight hydrocarbon compounds, no differences were found in any of the hydrocarbons present in cooked leg cuts in the current study, except pentylcyclopropane. Only the concentration of pentylcyclopropane was higher in the cuts injected with H2O than in other groups, yet there was no difference in the level of pentylcyclopropane in the cooked cuts from the two calcium-injected groups and control groups. In general, hydrocarbons may not be important contributors to meat flavor compared to other carbonyl compounds, which can be formed via either lipid oxidation or degradation of carotenoids.[Citation30]

There were no significant differences in the concentrations of ketone volatile compounds isolated in the cooked leg cuts. Ketone flavor compounds might be generated by either oxidation or thermal degradation of fatty acids or by the degradation of amino acids.[Citation26,Citation31] One furan, three alcohols, and one sulfur-derived flavor compounds were isolated in the cooked leg cuts; however, no differences were found in those flavor compounds among treatment groups in the present study. The 2-pentylfuran is produced by the oxidation of linolenic acid (C18:3n3).[Citation26,Citation31] Fatty acids and amino acids are precursors of variable volatile compounds. Alcohols are generally derived from oxidative degradation by lipoxygenase alone or in combination with a hydroperoxide lyase of precursors of volatile compounds.[Citation26] However, alcohols may not be important contributors to meat flavor compared to other carbonyl compounds. Carbon disulfide is a sulfur-containing volatile compound. Most of the sulfur compounds had low-odor threshold and were considered as major contributors to meat flavor.[Citation32] In early studies, H2S has been identified to characterize the volatile components of cooked red meat.[Citation30] However, H2S was not detected in the present study because of the limited molecular scan range (40–450). Since all meat products containing protein probably emanate H2S upon heating, the amount of H2S and its reaction with other compounds should highly impact cooked meat flavor.[Citation30,Citation32] Carbon disulfide may be formed from sulfur-containing amino acids (cysteine, cystine, and methionine) via reaction with free radicals.

Conclusion

The effect of CaCl2 injection on leg cuts from meat goats at 24 h postmortem was consistent with results from studies with other red meats using 0.2 M CaCl2 injection. While CaCl2 injection improved tenderness of goat leg cuts according to WBSF values, it was not perceived by the sensory panel. Furthermore, panelists were not able to detect off-flavor problems associated with CaCl2 injection. When CaCl2 was injected into goat leg cuts with the beef spice mixture, it resulted in a more desirable flavor. Calcium injection did not influence flavor volatile compounds in cooked chevon leg cuts. Results show that either CaCl2 or CaCl2 plus spice mix injection can be applied to improve tenderness of chevon without detrimental effects on palatability characteristics and flavor volatile compounds.

References

  • Putnam, J.;. Major Trends in U.S. Food Supply, 1909-1999. Food Review 2000, 23, 8–15.
  • James, N. A.; Berry, B. W. Use of Chevon in the Development of Low-Fat Meat Products. Journal of Animal Science 1997, 75, 571–577. DOI: 10.2527/1997.752571x.
  • McMillin, K. W.; Brock, A. P. Production Practices and Processing for Value-Added Goat Meat. Journal of Animal Science 2005, 83(E. Suppl.), E57–E68.
  • Kirton, A. H.;. Body and Carcass Composition and Meat Quality of the New Zealand Feral Goat (Capra Hircus). New Zealand Journal of Agricultural Research 1970, 13, 167–181. DOI: 10.1080/00288233.1970.10421206.
  • Hogg, B. W.; Mercer, G. J. K.; Mortimer, B. J.; Kirton, A. H.; Duganzich, D. M. Meat Yields and Chemical Composition of Muscle in New Zealand Goats. Proceedings of the New Zealand of Society of Animal Production 1989, 49, 153–156.
  • Smith, G. C.; Pike, M. I.; Carpenter, Z. L. Comparison of the Palatability of Goat Meat and Meat from Other Animal Species. Journal of Food Science 1974, 39, 1145–1146. DOI: 10.1111/j.1365-2621.1974.tb07338.x.
  • Griffin, C. L.; Orcutt, M. W.; Riley, R. R.; Smith, G. C.; Savell, J. W.; Shelton, M. Evaluation of Palatability of Lamb, Mutton, and Chevon by Sensory Panels of Various Cultural Backgrounds. Small Ruminant Research 1992, 8, 67–74. DOI: 10.1016/0921-4488(92)90008-R.
  • Miller, B.;. Understanding Consumers. Beef Today 1992, 8, 40.
  • Savell, J.; Shackelford, S. D. Significance of Tenderness to the Meat Industry. Proceedings of the Reciprocal Meat Conference 1992, 45, 43–46.
  • Wheeler, T. L.; Koohmaraie, M. Prerigor and Postrigor Changes in Tenderness of Ovine Longissimus Muscle. Journal of Animal Science 1994, 72, 1232–1238. DOI: 10.2527/1994.7251232x.
  • Kannan, G.; Chawan, C. B.; Kouakou, B.; Gelaye, S. Influence of Packing Method and Storage Time on Shear Value and Mechanical Strength of Intramuscular Connective Tissue of Chevon. Journal of Animal Science 2002, 80, 2383–2389.
  • Pringle, T. D.; Calkins, C. R.; Koohmaraie, M.; Jones, S. J. Effects over Time of Feeding a Beta-Adrenergic Agonist to Wether Lambs on Animal Performance, Muscle Growth, Endogenous Muscle Proteinase Activities, and Meat Tenderness. Journal of Animal Science 1993, 71, 636–644. DOI: 10.2527/1993.713636x.
  • Koohmaraie, M.; Shackelford, S. D.; Wheeler, T. L.; Lonergan, S. M.; Doumit, M. E. A Muscle Hypertrophy Condition in Lamb (Callipyge): Characterization of Effects on Muscle Growth and Meat Quality Traits. Journal of Animal Science 1995, 73, 3596–3607. DOI: 10.2527/1995.73123596x.
  • Koohmaraie, M.; Crouse, J. D.; Mersmann, H. J. Acceleration of Postmortem Tenderization in Ovine Carcasses through Infusion of Calcium Chloride: Effect of Concentration and Ionic Strength. Journal of Animal Science 1989, 67, 934–942. DOI: 10.2527/jas1989.674934x.
  • Koohmaraie, M.;. Quantification of Ca(2)+-Dependent Protease Activities by Hydrophobic and Ion-Exchange Chromatography. Journal of Animal Science 1990, 68, 659–665. DOI: 10.2527/1990.683659x.
  • Pringle, T. D.; Harrelson, J. M.; West, R. L.; Williams, S. E.; Johnson, D. D. Calcium-Activated Tenderization of Strip Loin, Top Loin, and Top around Steaks in Diverse Genotypes of Cattle. Journal of Animal Science 1999, 77, 3230–3237. DOI: 10.2527/1999.77123230x.
  • Morgan, J. B.; Miller, R. K.; Mendez, F. M.; Hale, D. S.; Savell, J. W. Using Calcium Chloride Injection to Improve Tenderness of Beef from Mature Cows. Journal of Animal Science 1991, 69, 3274–3283. DOI: 10.2527/1991.6983274x.
  • Wheeler, T. L.; Koohmaraie, M.; Lansdell, J. L.; Siragusa, G. R.; Miller, M. F. Effect of Postmortem Injection Time, Injection Level and Concentration of Calcium Chloride on Beef Quality Traits. Journal of Animal Science 1993, 71, 2965–2974. DOI: 10.2527/1993.71112965x.
  • Clare, T. L.; Jackson, S. P.; Miller, M. F.; Elliott, C. T.; Ramsey, C. B. Improving Tenderness of Normal and Callipyge Lambs with Calcium Chloride. Journal of Animal Science 1997, 75, 377–385. DOI: 10.2527/1997.752377x.
  • Olson, S.; McMillin, K. W.; Phelps, O.; Michel, M. E. Institutional Meat Purchase Specifications for Goat Carcasses and Meat. Journal of Animal Science 1999, 77(1), 14 (Abstracts).
  • Lee, J. H.; Kouakou, B.; Kannan, G. Chemical Composition and Quality Characteristics of Chevon from Goats Fed Different Post-Weaning Diets. Small Ruminant Research 2008, 75, 177–184. DOI: 10.1016/j.smallrumres.2007.10.003.
  • Cross, H. R.; Moen, R.; Stanfield, M. S. Training and Testing of Judges for Sensory Analysis of Meat Quality. Food Technology. 1978, 32, 48–54.
  • Harmon, A. D.;. Solid-Phase Microextraction for the Analysis of Flavors. In Techniques for Analyzing Food Aroma; Marshili, R., Ed.; Marcel Dekker: New York, NY, 1997; pp. 81–112.
  • Wheeler, T. L.; Koohmaraie, M.; Shackelford, S. D. Effect of Postmortem Injection Time and Postinjection Aging Time on the Calcium-Activated Tenderization Process in Beef. Journal of Animal Science 1997, 75, 2652–2660. DOI: 10.2527/1997.75102652x.
  • Melton, S. L.;. Effects of Feeds on Flavor of Red Meat: A Review. Journal of Animal Science 1990, 68, 4421–4435. DOI: 10.2527/1990.68124421x.
  • deMan, J. H.; John, M. Lipids. In Principle of Food Chemistry, 3rd ed.; Eds., deMan, J. H., John, M.; Spring-Verlag: New York, NY, 1999; pp. 33–110.
  • Caporaso, F.; Sink, J. D.; Dimick, P. S.; Mussinan, C. J.; Sanderson, A. Volatile Flavor Constituents of Ovine Adipose Tissue. Journal of Agricultural Food Chemistry 1977, 25, 1230–1233. DOI: 10.1021/jf60214a040.
  • Lamikanra, V. T.; Dupuy, H. P. Analysis of Volatiles Related to Warmed over Flavor of Cooked Chevon. Journal of Food Science 1990, 55, 861–862. DOI: 10.1111/jfds.1990.55.issue-3.
  • Pokorny, J.;. Flavor Chemistry of Deep Fat Frying in Oil. In Flavor Chemistry of Lipids Foods; Min, D. B., Smouse, T. H., Eds.; American Oil Chemistry Society: Champaign, IL, 1989; pp. 113–155.
  • Cramer, D. A.;. Chemical Compounds Implicated in Lamb Flavor. Food Technology. 1983, 37, 429–457.
  • Durnford, E.; Shahidi, F. Flavor of Fish Meat. In Flavor of Meat, Meat Products and Seafood, 2nd ed.; Ed., Shahidi, F.; Chapman & Hall: London, UK, 1998; pp. 131–158.
  • Szuhaj, B. F.; Sipos, E. F. Flavor Chemistry of Phospholipids. In Flavor Chemistry of Lipid Foods; Min, D. B., Smouse, T. H., Eds.; American Oil Chemistry Society: Champaign, IL, 1989; pp. 265–289.