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Journal of Biological Education Volume 54, 2020 - Issue 5
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Research Article

A simple, economical and effective ELISA using simulated serum sample for teaching the concept and application of antigen–antibody interactions in undergraduate laboratory

Latifa B. KhanDepartment of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New ZealandCorrespondence[email protected]
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Jia-Yun C. TsaiDepartment of Molecular Medicine and Pathology, School of Medical Sciences, The University of Auckland, Auckland, New ZealandView further author information
Pages 476-484 | Published online: 24 Apr 2019

References

  • Baker, W. P. and Moore C.R. 1996. “A simple exercise for undergraduate biology.” Teaching guide [for teacher (052)], College of Science and Allied Health, Grand Canyon University, Phoenix, AZ. Available online at Education Resources Information Center (ERIC Record No E396952).
  • Brokaw, A., and B. A. Cobb. 2009. “A Simple Test Tube-Based ELISA Experiment for the High-School Classroom.” Biochemistry and Molecular Biology Education: a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology 37 (4): 243–248. doi:10.1002/bmb.20308.
  • Gerbig, D. G., Jr., J. Fenk Christopher, and S. Goodhart Amy. 2000. “The Dot Blot ELISA: A Rapid & Simple Experiment to Demonstrate Antibody-Antigen Interactions.” The American Biology Teacher 62 (8): 583–587. doi:10.2307/4450982.
  • Giri, B., R. R. Peesara, N. Yanagisawa, and D. Dutta. 2015. “Undergraduate Laboratory Module for Implementing ELISA on the High Performance Microfluidic Platform.” Journal of Chemical Education 92 (4): 728–732. doi:10.1021/ed4009107.
  • Miller, H. B., D. Scott Witherow, and S. Carson. 2012. “Student Learning Outcomes and Attitudes When Biotechnology Lab Partners are of Different Academic Levels.” CBE Life Sciences Education 11 (3): 323–332. doi:10.1187/cbe.11-10-0094.
  • Olson, J., M. Amolins, and P. Vitiello. 2017. “Creating a Reliable, Cost-Effective ELISA Simulation.” The American Biology Teacher 79 (4): 301–304. doi:10.1525/abt.2017.79.4.301.
  • Ott, L. E., and S. Carson. 2014. “Immunological Tools: Engaging Students in the Use and Analysis of Flow Cytometry and Enzyme-Linked Immunosorbent Assay (ELISA).” Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology 42 (5): 382–397. doi:10.1002/bmb.20808.
  • Powers, J. L., K. D. Rippe, K. Imarhia, A. Swift, M. Scholten, and N. Islam. 2012. “A Direct, Competitive Enzyme-Linked Immunosorbent Assay (ELISA) as A Quantitative Technique for Small Molecules.” Journal of Chemical Education 89 (12): 1587–1590. doi:10.1021/ed2005505.
  • Robinson, D. L., and M. Lau Joann. 2012. “Assessment of an ELISA Laboratory Exercise.” The American Biology Teacher 74 (8): 558–563. doi:10.1525/abt.2012.74.8.5.
  • Song, Y. W., and E. H. Kang. 2010. “Autoantibodies in Rheumatoid Arthritis: Rheumatoid Factors and Anticitrullinated Protein Antibodies.” QJM: an International Journal of Medicine 103 (3): 139–146. doi:10.1093/qjmed/hcp165.

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