Base Modification and the Phosphoramidite Approach

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• All oligomers were synthesized at 0.2 umol scale on an Applied Bio‐systems 381A DNA Synthesizer with the same set of reagents all commercially available from Applied Biosystems (ABI) as of 9/1/86, with the exception that the 150‐mers were synthesized on a “wide pore” CPG support (1000A0, 18 umol/g deoxycytidine). Cycle‐2 (ABI User Bulletin No. 6, Revised 7/1/86) and Cycle‐1 (ABI User Bulletin No. 1, 11/1/85), were used for “2‐cyanoethyl” and “methyl” syntheses, respectively. Deprotections were performed manually: 1) Thiophenoxide solution (0.5 h, 25°C, methyl syntheses only) followed by cone NH, (aq) (53°C, 10–16h).
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• Cathcart , R. 3Manuscript in preparation
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