Structure-function effects of different pectin chemistries and its impact on the gastrointestinal immune barrier system

References

• Abbeele, P. V. D., S. Deyaert, R. Albers, A. Baudot, and A. Mercenier. 2023. Carrot RG-I reduces interindividual differences between 24 adults through consistent effects on gut microbiota composition and function ex vivo. Nutrients 15 (9):2090. doi:10.3390/nu15092090.
   PubMed Web of Science ®Google Scholar
• Abbeele, P. V. D., C. Duysburgh, I. Cleenwerck, R. Albers, M. Marzorati, and A. Mercenier. 2021. Consistent prebiotic effects of carrot Rg-I on the gut microbiota of four human adult donors in the shime® model despite baseline individual variability. Microorganisms 9 (10):2142. doi:10.3390/microorganisms9102142.
   PubMed Web of Science ®Google Scholar
• Ai, L., Y.-C. Chung, S.-Y. Lin, K.-C. Lee, P. F.-H. Lai, Y. Xia, G. Wang, and S. W. Cui. 2018. Active pectin fragments of high in vitro antiproliferation activities toward human colon adenocarcinoma cells: Rhamnogalacturonan II. Food Hydrocolloids. 83(October): 239–45. doi:10.1016/j.foodhyd.2018.05.017.
   Web of Science ®Google Scholar
• Allaire, J. M., S. M. Crowley, H. T. Law, S. Y. Chang, H. J. Ko, and B. A. Vallance. 2018. The intestinal epithelium: Central coordinator of mucosal immunity. Trends in Immunology 39 (9):677–96. doi:10.1016/j.it.2018.04.002.
   PubMed Web of Science ®Google Scholar
• Allaire, J. M., V. Morampudi, S. M. Crowley, M. Stahl, H. B. Yu, K. Bhullar, L. A. Knodler, B. Bressler, K. Jacobson, and B. A. Vallance. 2018. Frontline defenders: Goblet cell mediators dictate host-microbe interactions in the intestinal tract during health and disease. American Journal of Physiology. Gastrointestinal and Liver Physiology 314 (3):G360–G377. doi:10.1152/ajpgi.00181.2017.
   PubMed Web of Science ®Google Scholar
• Anderson, J. W., P. Baird, R. H. Davis, S. Ferreri, M. Knudtson, A. Koraym, V. Waters, and C. L. Williams. 2009. Health benefits of dietary fiber. Nutrition Reviews 67 (4):188–205. doi:10.1111/j.1753-4887.2009.00189.x.
   PubMed Web of Science ®Google Scholar
• Arrieta, M. C., K. Madsen, J. Doyle, and J. Meddings. 2009. Reducing small intestinal permeability attenuates colitis in the IL10 gene-deficient mouse. Gut 58 (1):41–8. doi:10.1136/gut.2008.150888.
   PubMed Web of Science ®Google Scholar
• Atuma, C., V. Strugala, A. Allen, and L. Holm. 2001. The adherent gastrointestinal mucus gel layer: Thickness and physical state in vivo. American Journal of Physiology - Gastrointestinal and Liver Physiology 280 (5):43–5.
   Web of Science ®Google Scholar
• Baggio, C. H., F. Lopes, A. Nascimento, T. Cipriani, D. McKay, and W. MacNaughton. 2017. Modulation of intestinal epithelial barrier function by rhamnogalacturonan. The FASEB Journal 31 (S1):995–7. doi:10.1096/fasebj.31.1_supplement.995.7.
   Google Scholar
• Bang, S. J., G. Kim, M. Y. Lim, E. J. Song, D. H. Jung, J. S. Kum, Y. D. Nam, C. S. Park, and D. H. Seo. 2018. The influence of in vitro pectin fermentation on the human fecal microbiome. AMB Express 8 (1):98. doi:10.1186/s13568-018-0629-9.
   PubMedGoogle Scholar
• Barber, T. M., S. Kabisch, A. F. H. Pfeiffer, and M. O. Weickert. 2020. The health benefits of dietary fibre. Nutrients 2020, Vol. 12, Page 320912 (10):3209. doi:10.3390/nu12103209.
   PubMed Web of Science ®Google Scholar
• Bayliss, C. E., and A. P. Houston. 1984. Characterization of plant polysaccharide- and mucin-fermenting anaerobic bacteria from human feces. Applied and Environmental Microbiology 48 (3):626–32. doi:10.1128/aem.48.3.626-632.1984.
   PubMed Web of Science ®Google Scholar
• Beukema, M., M. M. Faas, and P. De Vos. 2020. The effects of different dietary fiber pectin structures on the gastrointestinal immune barrier: Impact via gut microbiota and direct effects on immune cells. Experimental & Molecular Medicine 52 (9):1364–76. doi:10.1038/s12276-020-0449-2.
   PubMed Web of Science ®Google Scholar
• Beukema, M., É. Jermendi, M. A. van den Berg, M. M. Faas, H. A. Schols, and P. de Vos. 2021. The impact of the level and distribution of methyl-esters of pectins on TLR2-1 dependent anti-inflammatory responses. Carbohydrate Polymers 251(January): 117093. doi:10.1016/j.carbpol.2020.117093.
   PubMed Web of Science ®Google Scholar
• Beukema, M., É. Jermendi, M. M. P. Oerlemans, M. J. Logtenberg, R. Akkerman, R. An, M. A. van den Berg, E. G. Zoetendal, T. Koster, C. Kong, et al. 2022. The level and distribution of methyl-esters influence the impact of pectin on intestinal T cells, microbiota, and Ahr activation. Carbohydrate Polymers 286:119280. (June): doi:10.1016/j.carbpol.2022.119280.
   PubMed Web of Science ®Google Scholar
• Bianchi, F., N. Larsen, T. D. M. Tieghi, M. A. T. Adorno, W. Kot, S. M. I. Saad, L. Jespersen, and K. Sivieri. 2018. Modulation of gut microbiota from obese individuals by in vitro fermentation of citrus pectin in combination with bifidobacterium longum BB-46. Applied Microbiology and Biotechnology 102 (20):8827–40. doi:10.1007/s00253-018-9234-8.
   PubMed Web of Science ®Google Scholar
• Bischoff, S. C. 2011. Gut health: A new objective in medicine? BMC Medicine 9 (1):24. doi:10.1186/1741-7015-9-24.
   PubMedGoogle Scholar
• Blanco-Pérez, F., H. Steigerwald, S. Schülke, S. Vieths, M. Toda, and S. Scheurer. 2021. The dietary fiber pectin: Health benefits and potential for the treatment of allergies by modulation of gut microbiota. Current Allergy and Asthma Reports 21 (10):43. doi:10.1007/s11882-021-01020-z.
   PubMed Web of Science ®Google Scholar
• Bolognini, D., A. B. Tobin, G. Milligan, and C. E. Moss. 2016. The pharmacology and function of receptors for short-chain fatty acids. Molecular Pharmacology 89 (3):388–98. doi:10.1124/mol.115.102301.
   PubMed Web of Science ®Google Scholar
• Bourlioux, P., B. Koletzko, F. o Guarner, and V. Braesco. 2003. The intestine and its microflora are partners for the protection of the host: Report on the danone symposium ‘the intelligent intestine. The American Journal of Clinical Nutrition 78 (4):675–83. doi:10.1093/ajcn/78.4.675.
   PubMed Web of Science ®Google Scholar
• Braccini, I., and S. Pérez. 2001. Molecular basis of Ca2+-induced gelation in alginates and pectins: The egg-box model revisited. Biomacromolecules 2 (4):1089–96. doi:10.1021/bm010008g.
   PubMed Web of Science ®Google Scholar
• Brouns, F., E. Theuwissen, A. Adam, M. Bell, A. Berger, and R. P. Mensink. 2011. Cholesterol-lowering properties of different pectin types in mildly hyper-cholesterolemic men and women. European Journal of Clinical Nutrition 2012 66:566 (5):591–9. doi:10.1038/ejcn.2011.208.
   PubMed Web of Science ®Google Scholar
• Brown, L., B. Rosner, W. W. Willett, and F. M. Sacks. 1999. Cholesterol-lowering effects of dietary fiber: A meta-analysis. The American Journal of Clinical Nutrition 69 (1):30–42. doi:10.1093/ajcn/69.1.30.
   PubMed Web of Science ®Google Scholar
• Buhner, S., C. Buning, J. Genschel, K. Kling, D. Herrmann, A. Dignass, I. Kuechler, S. Krueger, H. H.-J. Schmidt, and H. Lochs. 2006. Genetic basis for increased intestinal permeability in families with Crohn’s disease: Role of CARD15 3020insC mutation? Gut 55 (3):342–7. doi:10.1136/gut.2005.065557.
   PubMed Web of Science ®Google Scholar
• Caffall, K. H., and D. Mohnen. 2009. The structure, function, and biosynthesis of plant cell wall pectic polysaccharides. Carbohydrate Research 344 (14):1879–900. doi:10.1016/j.carres.2009.05.021.
   PubMed Web of Science ®Google Scholar
• Cardoso, S. M., M. A. Coimbra, and J. A. Lopes da Silva. 2003. Temperature dependence of the formation and melting of pectin–Ca2+ networks: A rheological study. Food Hydrocolloids. 17 (6):801–7. doi:10.1016/S0268-005X(03)00101-2.
   Web of Science ®Google Scholar
• Cerf-Bensussan, N., and V. Gaboriau-Routhiau. 2010. The immune system and the gut microbiota: Friends or foes? Nature Reviews. Immunology 10 (10):735–44. doi:10.1038/nri2850.
   PubMed Web of Science ®Google Scholar
• Chan, S. Y., W. S. Choo, D. J. Young, and X. J. Loh. 2017. Pectin as a rheology modifier: Origin, structure, commercial production and rheology. Carbohydrate Polymers 161:118–39. doi:10.1016/j.carbpol.2016.12.033.
   PubMed Web of Science ®Google Scholar
• Chanput, W., J. Mes, R. A. M. Vreeburg, H. F. J. Savelkoul, and H. J. Wichers. 2010. Transcription profiles of LPS-stimulated THP-1 monocytes and macrophages: A tool to study inflammation modulating effects of food-derived compounds. Food & Function 1 (3):254–61. 2010. doi:10.1039/c0fo00113a.
   PubMed Web of Science ®Google Scholar
• Christensen, S. H. 2020. Pectins. Food Hydrocolloids. 205–30.
   Google Scholar
• Chung, W. S., Faith, A. W. Walker, P. Louis, J. Parkhill, J. Vermeiren, D. Bosscher, S. H. Duncan, and H. J. Flint. 2016. Modulation of the human gut microbiota by dietary fibres occurs at the species level. BMC Biology 14 (1):3. doi:10.1186/s12915-015-0224-3.
   PubMedGoogle Scholar
• Ciriminna, R., N. Chavarría-Hernández, A. I. R. Hernández, and M. Pagliaro. 2015. Pectin: A new perspective from the biorefinery standpoint. Biofuels, Bioproducts and Biorefining 9 (4):368–77. doi:10.1002/bbb.1551.
   Web of Science ®Google Scholar
• Cui, Y. S., Y. X. Li, S. L. Jiang, A. N. Song, Z. Fu, C. X. Dong, Z. Yao, and W. Qiao. 2020. Isolation, purification, and structural characterization of polysaccharides from atractylodis macrocephalae rhizoma and their immunostimulatory activity in RAW264.7 cells. International Journal of Biological Macromolecules 163(November): 270–8. doi:10.1016/j.ijbiomac.2020.06.269.
   PubMed Web of Science ®Google Scholar
• Decreux, A., and J. Messiaen. 2005. Wall-associated kinase WAK1 interacts with cell wall pectins in a calcium-induced conformation. Plant and Cell Physiology 46 (2):268–78. doi:10.1093/pcp/pci026.
   PubMed Web of Science ®Google Scholar
• Dongowski, G., and A. Lorenz. 2004. Intestinal steroids in rats are influenced by the structural parameters of pectin. The Journal of Nutritional Biochemistry 15 (4):196–205. doi:10.1016/S0955-2863(03)00080-9.
   PubMed Web of Science ®Google Scholar
• Dongowski, G., A. Lorenz, and H. Anger. 2000. Degradation of pectins with different degrees of esterification by bacteroides thetaiotaomicron isolated from human gut flora. Applied and Environmental Microbiology 66 (4):1321–7. doi:10.1128/AEM.66.4.1321-1327.2000.
   PubMed Web of Science ®Google Scholar
• Dongowski, G., A. Lorenz, and J. Proll. 2002. The degree of methylation influences the degradation of pectin in the intestinal tract of rats and in vitro. The Journal of Nutrition 132 (7):1935–44. doi:10.1093/jn/132.7.1935.
   PubMed Web of Science ®Google Scholar
• Dong, Q., X. Liu, J. Yao, X. T. Dong, C. Ma, Y. X. Xu, J. N. Fang, and K. Ding. 2010. Structural characterization of a pectic polysaccharide from nerium indicum flowers. Phytochemistry 71 (11-12):1430–7. doi:10.1016/j.phytochem.2010.05.019.
   PubMed Web of Science ®Google Scholar
• do Prado, S. B. R., G. R. Santos, P. A. Mourão, and J. P. Fabi. Chelate-soluble pectin fraction from papaya pulp interacts with galectin-3 and inhibits colon cancer cell proliferation. International Journal of Biological Macromolecules 126:170–8. doi:10.1016/j.ijbiomac.2018.12.191.
   PubMed Web of Science ®Google Scholar
• Dumic, J., S. Dabelic, and M. Flögel. 2006. Galectin-3: An open-ended story. Biochimica Et Biophysica Acta 1760 (4):616–35. doi:10.1016/j.bbagen.2005.12.020.
   PubMed Web of Science ®Google Scholar
• Edelblum, K. L., and J. R. Turner. 2009. The tight junction in inflammatory disease: Communication breakdown. Current Opinion in Pharmacology 9 (6):715–20. doi:10.1016/j.coph.2009.06.022.
   PubMed Web of Science ®Google Scholar
• Elshahed, M. S., A. Miron, A. C. Aprotosoaie, and M. A. Farag. 2021. Pectin in diet: Interactions with the human microbiome, role in gut homeostasis, and nutrient-drug interactions. Carbohydrate Polymers 255 (March):117388. doi:10.1016/j.carbpol.2020.117388.
   PubMedGoogle Scholar
• Ermund, A., A. Schütte, M. E. V. Johansson, J. K. Gustafsson, and G. C. Hansson. 2013. Studies of mucus in mouse stomach, small intestine, and colon. I. Gastrointestinal mucus layers have different properties depending on location as well as over the peyer’s patches. American Journal of Physiology - Gastrointestinal and Liver Physiology 305 (5):341–7.
   PubMed Web of Science ®Google Scholar
• Everard, A., and P. D. Cani. 2013. Diabetes, obesity and gut microbiota. Best Practice & Research. Clinical Gastroenterology 27 (1):73–83. doi:10.1016/j.bpg.2013.03.007.
   PubMed Web of Science ®Google Scholar
• Faderl, M., M. Noti, N. Corazza, and C. Mueller. 2015. Keeping bugs in check: The mucus layer as a critical component in maintaining intestinal homeostasis. IUBMB Life 67 (4):275–85. doi:10.1002/iub.1374.
   PubMed Web of Science ®Google Scholar
• Fan, L. L., S. Zuo, H. Z. Tan, J. L. Hu, J. B. Cheng, Q. Y. Wu, and S. P. Nie. 2020. Preventive effects of pectin with various degrees of esterification on ulcerative colitis in mice. Food & Function 11 (4):2886–97. doi:10.1039/c9fo03068a.
   PubMed Web of Science ®Google Scholar
• Forchielli, M. L., and W. A. Walker. 2005. The role of gut-associated lymphoid tissues and mucosal defence. The British Journal of Nutrition 93 Suppl 1 (S1):S41–S48. doi:10.1079/bjn20041356.
   PubMedGoogle Scholar
• Garcia-Diez, F., V. Garcia-Mediavilla, J. E. Bayon, and J. Gonzalez-Gallego. 1996. Pectin feeding influences fecal bile acid excretion, hepatic bile acid and cholesterol synthesis and serum cholesterol in rats. The Journal of Nutrition 126 (7):1766–71.
   PubMed Web of Science ®Google Scholar
• Girotti, M. R., M. Salatino, T. Dalotto-Moreno, and G. A. Rabinovich. 2019. Sweetening the hallmarks of cancer: Galectins as multifunctional mediators of tumor progression. Journal of Experimental Medicine 217 (2):e20182041. doi:10.1084/jem.20182041.
   Web of Science ®Google Scholar
• Gómez, B., B. Gullón, C. Remoroza, H. A. Schols, J. C. Parajó, and J. L. Alonso. 2014. Purification, characterization, and prebiotic properties of pectic oligosaccharides from orange peel wastes. Journal of Agricultural and Food Chemistry 62 (40):9769–82. doi:10.1021/jf503475b.
   PubMed Web of Science ®Google Scholar
• Goto, Y., and H. Kiyono. 2012. Epithelial barrier: An interface for the cross-communication between gut flora and immune system. Immunological Reviews 245 (1):147–63. doi:10.1111/j.1600-065X.2011.01078.x.
   PubMed Web of Science ®Google Scholar
• Grønhaug, T. E., H. Kiyohara, A. Sveaass, D. Diallo, H. Yamada, and B. S. Paulsen. 2011. Beta-d-(1 → 4)-galactan-containing side chains in RG-I regions of pectic polysaccharides from Biophytum Petersianum Klotzsch. contribute to expression of immunomodulating activity against intestinal Peyer’s patch cells and macrophages. Phytochemistry 72 (17):2139–47. doi:10.1016/j.phytochem.2011.08.011.
   PubMed Web of Science ®Google Scholar
• Gulfi, M., E. Arrigoni, and R. Amadò. 2006. The chemical characteristics of apple pectin influence its fermentability in vitro. LWT - Food Science and Technology 39 (9):1001–4. doi:10.1016/j.lwt.2006.02.013.
   Web of Science ®Google Scholar
• Gunning, A. P., R. J. M. Bongaerts, and V. J. Morris. 2009. Recognition of galactan components of pectin by galectin-3. FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology 23 (2):415–24. doi:10.1096/fj.08-106617.
   PubMed Web of Science ®Google Scholar
• Hino, S., K. Sonoyama, H. Bito, H. Kawagishi, S. Aoe, and T. Morita. 2013. Low-methoxyl pectin stimulates small intestinal mucin secretion irrespective of goblet cell proliferation and is characterized by jejunum Muc2 upregulation in rats. The Journal of Nutrition 143 (1):34–40. doi:10.3945/jn.112.167064.
   PubMed Web of Science ®Google Scholar
• Ho, G. T. T., Y. F. Zou, H. Wangensteen, and H. Barsett. 2016. RG-I regions from elderflower pectins substituted on GalA are strong immunomodulators. International Journal of Biological Macromolecules 92(November): 731–8. doi:10.1016/j.ijbiomac.2016.07.090.
   PubMed Web of Science ®Google Scholar
• Hounnou, G., C. Destrieux, J. Desmé, P. Bertrand, and S. Velut. 2002. Anatomical study of the length of the human intestine. Surgical and Radiologic Anatomy: SRA 24 (5):290–4. doi:10.1007/s00276-002-0057-y.
   PubMed Web of Science ®Google Scholar
• Hsu, D. K., R. Y. Yang, Z. Pan, L. Yu, D. R. Salomon, W. P. Fung-Leung, and F. T. Liu. 2000. Targeted disruption of the galectin-3 gene results in attenuated peritoneal inflammatory responses. The American Journal of Pathology 156 (3):1073–83. doi:10.1016/S0002-9440(10)64975-9.
   PubMed Web of Science ®Google Scholar
• Huang, W. Q., Q. Y. Fang, L. L. Fan, T. Hong, H. Z. Tan, and S. P. Nie. 2022. Pectin with Various degrees of esterification differentially alters gut microbiota and metabolome of healthy adults. eFood 3 (1-2):e5. doi:10.1002/efd2.5.
   Google Scholar
• Hu, R., Y. Xu, C. J. Yu, K. He, Q. Tang, C. L. Jia, G. He, X. Y. Wang, Y. Z. Kong, and G. K. Zhou. 2017. Transcriptome analysis of genes involved in secondary cell wall biosynthesis in developing internodes of miscanthus lutarioriparius. Scientific Reports 7 (1):9034. doi:10.1038/s41598-017-08690-8.
   PubMed Web of Science ®Google Scholar
• Hu, S. X., R. Kuwabara, M. Beukema, M. Ferrari, B. J. De Haan, M. T. Walvoort, P. De Vos, and A. M. Smink. 2020. Low methyl-esterified pectin protects pancreatic β-cells against diabetes-induced oxidative and inflammatory stress via galectin-3. Carbohydrate Polymers 249(December): 116863. doi:10.1016/j.carbpol.2020.116863.
   PubMed Web of Science ®Google Scholar
• Ishisono, K., T. Yabe, and K. Kitaguchi. 2017. Citrus pectin attenuates endotoxin shock via suppression of toll-like receptor signaling in Peyer’s patch myeloid cells. The Journal of Nutritional Biochemistry 50(December): 38–45. doi:10.1016/j.jnutbio.2017.07.016.
   PubMed Web of Science ®Google Scholar
• Jiang, T. T., X. J. Gao, C. Wu, F. Tian, Q. C. Lei, J. C. Bi, B. X. Xie, H. Y. Wang, S. Chen, and X. Y. Wang. 2016. Apple-derived pectin modulates gut microbiota, improves gut barrier function, and attenuates metabolic endotoxemia in rats with diet-induced obesity. Nutrients 8 (3):126. doi:10.3390/nu8030126.
   PubMed Web of Science ®Google Scholar
• Jia, Z. Q., H. D. Tan, W. Chen, Q. S. Liu, G. J. Yang, and K. K. Li. 2018. Pectin oligosaccharides ameliorate colon cancer by regulating oxidative stress- and inflammation-activated signaling pathways 9: 1504.
   Google Scholar
• Johansson, M. E. V., D. Ambort, T. Pelaseyed, A. Schütte, J. K. Gustafsson, A. Ermund, D. B. Subramani, J. M. Holmén-Larsson, K. A. Thomsson, J. H. Bergström, et al. 2011. Composition and functional role of the mucus layers in the intestine. Cellular and Molecular Life Sciences: CMLS 68 (22):3635–41. doi:10.1007/s00018-011-0822-3.
   PubMed Web of Science ®Google Scholar
• Johansson, M. E., J. M. H. Larsson, and G. C. Hansson. 2011. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host–microbial interactions. Proceedings of the National Academy of Sciences of the United States of America 108 Suppl 1 (Suppl 1):4659–65. doi:10.1073/pnas.1006451107.
   PubMedGoogle Scholar
• Kagnoff, M. F. 1993. Immunology of the intestinal tract. Gastroenterology 105 (5):1275–80. doi:10.1016/0016-5085(93)90128-y.
   PubMed Web of Science ®Google Scholar
• Kamada, N., S. U. Seo, G. Y. Chen, and G. Núñez. 2013. Role of the gut microbiota in immunity and inflammatory disease. Nature Reviews. Immunology 13 (5):321–35. doi:10.1038/nri3430.
   PubMed Web of Science ®Google Scholar
• Kayama, H., and K. Takeda. 2012. Regulation of intestinal homeostasis by innate and adaptive immunity. International Immunology 24 (11):673–80. doi:10.1093/intimm/dxs094.
   PubMed Web of Science ®Google Scholar
• Kelly, D., and I. E. Mulder. 2012. Microbiome and immunological interactions. Nutrition Reviews 70 Suppl 1 (suppl_1):S18–S30. doi:10.1111/j.1753-4887.2012.00498.x.
   PubMedGoogle Scholar
• Kim, C. H., and J. Betz. 2018. Immune regulation by microbiome metabolites. Immunology 154 (2):220–9. doi:10.1111/imm.12930.
   PubMed Web of Science ®Google Scholar
• Kim, S., A. Covington, and E. G. Pamer. 2017. The intestinal microbiota: Antibiotics, colonization resistance, and enteric pathogens. Immunological Reviews 279 (1):90–105. doi:10.1111/imr.12563.
   PubMed Web of Science ®Google Scholar
• Koh, A., F. De Vadder, P. Kovatcheva-Datchary, and F. Bäckhed. 2016. From dietary fiber to host physiology: Short-chain fatty acids as key bacterial metabolites. Cell 165 (6):1332–45. doi:10.1016/j.cell.2016.05.041.
   PubMed Web of Science ®Google Scholar
• Kolatsi-Joannou, M., K. L. Price, P. J. Winyard, and D. A. Long. 2011. Modified citrus pectin reduces galectin-3 expression and disease severity in experimental acute kidney injury. PLoS One 6 (4):e18683. doi:10.1371/journal.pone.0018683.
   PubMed Web of Science ®Google Scholar
• König, J., J. Wells, P. D. Cani, C. L. García-Ródenas, T. MacDonald, A. Mercenier, J. Whyte, F. Troost, and R. J. Brummer. 2016. Human intestinal barrier function in health and disease. Clinical and Translational Gastroenterology 7 (10):e196. doi:10.1038/ctg.2016.54.
   PubMed Web of Science ®Google Scholar
• Kontogiorgos, V. 2020. Pectin: Technological and Physiological Properties. Pectin: Technological and Physiological Properties, October, 1–207.
   Google Scholar
• Kootte, R. S., A. Vrieze, F. Holleman, G. M. Dallinga-Thie, E. G. Zoetendal, W. M. de Vos, A. K. Groen, J. B. Hoekstra, E. S. Stroes, and M. Nieuwdorp. 2012. The therapeutic potential of manipulating gut microbiota in obesity and type 2 diabetes mellitus. Diabetes, Obesity & Metabolism 14 (2):112–20. doi:10.1111/j.1463-1326.2011.01483.x.
   PubMed Web of Science ®Google Scholar
• Larsen, N., C. B. De Souza, L. Krych, T. B. Cahú, M. Wiese, W. Kot, K. M. Hansen, A. Blennow, K. Venema, and L. Jespersen. 2019. Potential of pectins to beneficially modulate the gut microbiota depends on their structural properties. Frontiers in Microbiology 10(FEB): 223. doi:10.3389/fmicb.2019.00223.
   PubMed Web of Science ®Google Scholar
• Lattimer, J. M., and M. D. Haub. 2010. Effects of dietary fiber and its components on metabolic health. Nutrients 2 (12):1266–89. doi:10.3390/nu2121266.
   PubMed Web of Science ®Google Scholar
• Lee, Y. K., and S. K. Mazmanian. 2010. Has the microbiota played a critical role in the evolution of the adaptive immune system? Science (New York, N.Y.) 330 (6012):1768–73. doi:10.1126/science.1195568.
   PubMed Web of Science ®Google Scholar
• Lerouge, P., M. A. O’Neill, A. G. Darvill, and P. Albersheim. 1993. Structural characterization of endo-glycanase-generated oligoglycosyl side chains of rhamnogalacturonan I. Carbohydrate Research 243 (2):359–71. doi:10.1016/0008-6215(93)87039-u.
   PubMed Web of Science ®Google Scholar
• Liu, L. S., M. L. Fishman, K. B. Hicks, and M. Kende. 2005. Interaction of various pectin formulations with porcine colonic tissues. Biomaterials 26 (29):5907–16. doi:10.1016/j.biomaterials.2005.03.005.
   PubMed Web of Science ®Google Scholar
• Liu, Y. L., M. Dong, Z. Y. Yang, and S. Y. Pan. 2016. Anti-diabetic effect of citrus pectin in diabetic rats and potential mechanism via PI3K/Akt signaling pathway. International Journal of Biological Macromolecules 89(August): 484–8. doi:10.1016/j.ijbiomac.2016.05.015.
   PubMed Web of Science ®Google Scholar
• Li, W. F., K. Zhang, and H. Y. Yang. 2018. Pectin alleviates high fat (lard) diet-induced nonalcoholic fatty liver disease in mice: Possible role of short-chain fatty acids and gut microbiota regulated by pectin. Journal of Agricultural and Food Chemistry 66 (30):8015–25. doi:10.1021/acs.jafc.8b02979.
   PubMed Web of Science ®Google Scholar
• Li, X., J. Jiang, S. Shi, S. W. A. Bligh, Y. Li, Y. Jiang, D. Huang, Y. Ke, and S. Wang. 2014. A RG-II type polysaccharide purified from aconitum coreanum alleviates lipopolysaccharide-induced inflammation by inhibiting the NF-KB signal pathway. PLoS One 9 (6):e99697. doi:10.1371/journal.pone.0099697.
   PubMed Web of Science ®Google Scholar
• Lutter, R., A. Teitsma-Jansen, E. Floris, S. Lone-Latif, A. Ravi, Y. S. Sabogal Pineros, T. Dekker, B. Smids, R. Khurshid, M. Aparicio-Vergara, et al. 2021. The dietary intake of carrot‐derived rhamnogalacturonan‐I accelerates and augments the innate immune and anti‐viral interferon response to rhinovirus infection and reduces duration and severity of symptoms in humans in a randomized trial. Nutrients 13 (12):4395. doi:10.3390/nu13124395.
   PubMed Web of Science ®Google Scholar
• Macfarlane, S., and G. T. Macfarlane. 2003. Regulation of short-chain fatty acid production. The Proceedings of the Nutrition Society 62 (1):67–72. doi:10.1079/PNS2002207.
   PubMed Web of Science ®Google Scholar
• Maloy, K. J., and F. Powrie. 2011. Intestinal homeostasis and its breakdown in inflammatory bowel disease. Nature 474 (7351):298–306. doi:10.1038/nature10208.
   PubMed Web of Science ®Google Scholar
• Man, A. L., M. E. Prieto-Garcia, and C. Nicoletti. 2004. Improving M cell mediated transport across mucosal barriers: Do certain bacteria hold the keys? Immunology 113 (1):15–22. doi:10.1111/j.1365-2567.2004.01964.x.
   PubMed Web of Science ®Google Scholar
• Mao, G. Z., S. Li, C. Orfila, X. M. Shen, S. Y. Zhou, R. J. Linhardt, X. Q. Ye, and S. G. Chen. 2019. Depolymerized RG-I-enriched pectin from citrus segment membranes modulates gut microbiota, increases SCFA production, and promotes the growth of Bifidobacterium Spp., Lactobacillus Spp. and Faecalibaculum Spp. Food & Function 10 (12):7828–43. doi:10.1039/c9fo01534e.
   PubMed Web of Science ®Google Scholar
• Maria-Ferreira, D., A. M. Nascimento, T. R. Cipriani, A. P. Santana-Filho, P. D. S. Watanabe, D. D. M. G. S. Ana, F. B. Luciano, K. C. P. Bocate, R. M. Van, d Wijngaard, et al. 2018. Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human caco-2 cells. Scientific Reports 8 (1):12261. doi:10.1038/s41598-018-30526-2.
   PubMed Web of Science ®Google Scholar
• Maria-Ferreira, D., L. M. da Silva, D. A. G. B. Mendes, D. d A. Cabrini, A. M. Nascimento, M. Iacomini, T. R. Cipriani, A. R. S. Santos, M. F. d P. Werner, and C. H. Baggio. 2014. Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: Gastroprotective and ulcer healing properties in rats. PLoS One 9 (1):e84762. doi:10.1371/journal.pone.0084762.
   PubMed Web of Science ®Google Scholar
• Marques, F. Z., E. Nelson, P.-Y. Chu, D. Horlock, A. Fiedler, M. Ziemann, J. K. Tan, S. Kuruppu, N. W. Rajapakse, A. El-Osta, et al. 2017. High-fiber diet and acetate supplementation change the gut microbiota and prevent the development of hypertension and heart failure in hypertensive mice. Circulation 135 (10):964–77. doi:10.1161/CIRCULATIONAHA.116.024545.
   PubMed Web of Science ®Google Scholar
• Marques, R., and I. G. Boneca. 2011. Expression and functional importance of innate immune receptors by intestinal epithelial cells. Cellular and Molecular Life Sciences: CMLS 68 (22):3661–73. doi:10.1007/s00018-011-0829-9.
   PubMed Web of Science ®Google Scholar
• May, C. D. 1990. Industrial pectins: Sources, production and applications. Carbohydrate Polymers 12 (1):79–99. doi:10.1016/0144-8617(90)90105-2.
   Web of Science ®Google Scholar
• McKay, S., P. Oranje, J. Helin, J. H. Koek, E. Kreijveld, P. van den Abbeele, U. Pohl, G. Bothe, M. Tzoumaki, M. Aparicio-Vergara, et al. 2021. Development of an affordable, sustainable and efficacious plant-based immunomodulatory food ingredient based on Bell pepper or carrot RG-I pectic polysaccharides. Nutrients 13 (3):963. doi:10.3390/nu13030963.
   PubMed Web of Science ®Google Scholar
• Metwaly, A., S. Reitmeier, and D. Haller. 2022. Microbiome risk profiles as biomarkers for inflammatory and metabolic disorders. Nature Reviews. Gastroenterology & Hepatology 19 (6):383–97. doi:10.1038/s41575-022-00581-2.
   PubMed Web of Science ®Google Scholar
• Min, B., I. Y. Bae, H. G. Lee, S. H. Yoo, and S. Lee. 2010. Utilization of pectin-enriched materials from apple pomace as a fat replacer in a model food system. Bioresource Technology 101 (14):5414–8. doi:10.1016/j.biortech.2010.02.022.
   PubMed Web of Science ®Google Scholar
• Mohnen, D. 2008. Pectin structure and biosynthesis. Current Opinion in Plant Biology 11 (3):266–77. doi:10.1016/j.pbi.2008.03.006.
   PubMed Web of Science ®Google Scholar
• Mörbe, U. M., P. B. Jørgensen, T. M. Fenton, N. V. Burg, L. B. Riis, J. Spencer, and W. W. Agace. 2021. Human gut-associated lymphoid tissues (GALT); Diversity, structure, and function. Mucosal Immunology 14 (4):793–802. doi:10.1038/s41385-021-00389-4.
   PubMed Web of Science ®Google Scholar
• Mort, A. J., F. Qiu, and N. O. Maness. 1993. Determination of the pattern of methyl esterification in pectin. Distribution of contiguous nonesterified residues. Carbohydrate Research 247 (C):21–35. doi:10.1016/0008-6215(93)84238-2.
   PubMedGoogle Scholar
• Mowat, A. M., and W. W. Agace. 2014. Regional specialization within the intestinal immune system. Nature Reviews. Immunology 14 (10):667–85. doi:10.1038/nri3738.
   PubMed Web of Science ®Google Scholar
• Naqash, F., F. A. Masoodi, S. A. Rather, S. M. Wani, and A. Gani. 2017. Emerging concepts in the nutraceutical and functional properties of pectin—A review. Carbohydrate Polymers 168:227–39. doi:10.1016/j.carbpol.2017.03.058.
   PubMed Web of Science ®Google Scholar
• Nascimento, A. M., L. M. de Souza, C. H. Baggio, M. F. d P. Werner, D. Maria-Ferreira, L. M. da Silva, G. L. Sassaki, P. A. J. Gorin, M. Iacomini, and T. R. Cipriani. 2013. Gastroprotective effect and structure of a rhamnogalacturonan from Acmella oleracea. Phytochemistry 85 (January):137–42. doi:10.1016/j.phytochem.2012.08.024.
   PubMedGoogle Scholar
• do Nascimento, G. E., S. M. B. Winnischofer, M. I. Ramirez, M. Iacomini, and L. M. C. Cordeiro. 2017. The influence of sweet pepper pectin structural characteristics on cytokine secretion by THP-1 macrophages. Food Research International (Ottawa, Ont.) 102(December): 588–94. doi:10.1016/j.foodres.2017.09.037.
   PubMed Web of Science ®Google Scholar
• Ndeh, D., A. Rogowski, A. Cartmell, A. S. Luis, A. Baslé, J. Gray, I. Venditto, J. Briggs, X. Zhang, A. Labourel, et al. 2017. Complex pectin metabolism by gut bacteria reveals novel catalytic functions. Nature 544 (7648):65–70. doi:10.1038/nature21725.
   PubMed Web of Science ®Google Scholar
• Neu, J., R. Sharma, and C. Young. 2010. Molecular modulation of intestinal epithelial barrier: Contribution of microbiota. Journal of Biomedicine & Biotechnology 2010:305879. 2010. doi:10.1155/2010/305879.
   PubMedGoogle Scholar
• Nishida, A., R. Inoue, O. Inatomi, S. Bamba, Y. Naito, and A. Andoh. 2017. Gut microbiota in the pathogenesis of inflammatory bowel disease. Clinical Journal of Gastroenterology 2017 11:1, 11 (1):1–10. doi:10.1007/s12328-017-0813-5.
   PubMed Web of Science ®Google Scholar
• Ohira, H., W. Tsutsui, and Y. Fujioka. 2017. Are short chain fatty acids in gut microbiota defensive players for inflammation and atherosclerosis? Journal of Atherosclerosis and Thrombosis 24 (7):660–72. doi:10.5551/jat.RV17006.
   PubMed Web of Science ®Google Scholar
• Okumura, R., and K. Takeda. 2018. Maintenance of intestinal homeostasis by mucosal barriers. Inflammation and Regeneration 38 (1):5. doi:10.1186/s41232-018-0063-z.
   PubMedGoogle Scholar
• Paone, P., and P. D. Cani. 2020. Mucus barrier, mucins and gut microbiota: The expected slimy partners? Gut 69 (12):2232–43. doi:10.1136/gutjnl-2020-322260.
   PubMed Web of Science ®Google Scholar
• Pappolla, M. A., G. Perry, X. Fang, M. Zagorski, K. Sambamurti, and B. Poeggeler. 2021. Indoles as essential mediators in the gut-brain axis. Their role in Alzheimer’s disease. Neurobiology of Disease 156(August): 105403. doi:10.1016/j.nbd.2021.105403.
   PubMed Web of Science ®Google Scholar
• Parkar, S. G., E. L. Redgate, R. Wibisono, X. X. Luo, E. T. Koh, and R. Schröder. 2010. Gut health benefits of kiwifruit pectins: Comparison with commercial functional polysaccharides. Journal of Functional Foods 2 (3):210–8. doi:10.1016/j.jff.2010.04.009.
   Web of Science ®Google Scholar
• Park, S. N., K. T. Noh, Y.-I. Jeong, I. D. Jung, H. K. Kang, G. S. Cha, S. J. Lee, J. K. Seo, D. H. Kang, T.-H. Hwang, et al. 2013. Rhamnogalacturonan II is a toll-like receptor 4 agonist that inhibits tumor growth by activating dendritic cell-mediated CD8+ T cells. Experimental & Molecular Medicine 2013 45:2 45 (2):e8–e8–e8. doi:10.1038/emm.2013.14.
   Google Scholar
• Perrone, P., C. M. Hewage, A. R. Thomson, K. Bailey, I. H. Sadler, and S. C. Fry. 2002. Patterns of methyl and O-acetyl esterification in Spinach pectins: New complexity. Phytochemistry 60 (1):67–77. doi:10.1016/s0031-9422(02)00039-0.
   PubMed Web of Science ®Google Scholar
• Peterson, L. W., and D. Artis. 2014. Intestinal epithelial cells: Regulators of barrier function and immune homeostasis. Nature Reviews. Immunology 14 (3):141–53. doi:10.1038/nri3608.
   PubMed Web of Science ®Google Scholar
• Petruzziello, L., F. Iacopini, M. Bulajic, S. Shah, and G. Costamagna. 2006. Review article: Uncomplicated diverticular disease of the colon. Alimentary Pharmacology & Therapeutics 23 (10):1379–91. doi:10.1111/j.1365-2036.2006.02896.x.
   PubMed Web of Science ®Google Scholar
• Popov, S. V., P. A. Markov, G. Y. Popova, I. R. Nikitina, L. Efimova, and Y. S. Ovodov. 2013. Anti-inflammatory activity of low and high methoxylated citrus pectins. Biomedicine & Preventive Nutrition 3 (1):59–63. doi:10.1016/j.bionut.2012.10.008.
   Google Scholar
• Popov, S. V., R. G. Ovodova, P. A. Markov, I. R. Nikitina, and Y. S. Ovodov. 2006. Protective effect of comaruman, a pectin of cinquefoil Comarum palustre L., on acetic acid-induced colitis in mice. Digestive Diseases and Sciences 51 (9):1532–7. doi:10.1007/s10620-005-9034-8.
   PubMed Web of Science ®Google Scholar
• Popov, S. V., and Y. S. Ovodov. 2013. Polypotency of the immunomodulatory effect of pectins. Biochemistry. Biokhimiia 78 (7):823–35. doi:10.1134/S0006297913070134.
   PubMed Web of Science ®Google Scholar
• Priyadarshini, M., K. Lednovich, K. Xu, S. Gough, B. Wicksteed, and B. T. Layden. 2021. FFAR from the gut microbiome crowd: SCFA receptors in T1D pathology. Metabolites 11 (5):302. doi:10.3390/metabo11050302.
   PubMed Web of Science ®Google Scholar
• Rajamma, S. S., V. Krishnaswami, and R. Kandasamy. 2022. Functional role of prebiotic supplement in brain signalling. Microbiome-Gut-Brain Axis: Implications on Health 215–36. Singapore: Springer. doi:10.1007/978-981-16-1626-6_9.
   Google Scholar
• Ralet, M. C., J. C. Cabrera, E. Bonnin, B. Quéméner, P. Hellìn, and J. F. Thibault. 2005. Mapping sugar beet pectin acetylation pattern. Phytochemistry 66 (15):1832–43. doi:10.1016/j.phytochem.2005.06.003.
   PubMed Web of Science ®Google Scholar
• Ralet, M. C., M. J. Crépeau, H. C. Buchholt, and J. F. Thibault. 2003. Polyelectrolyte behaviour and calcium binding properties of sugar beet pectins differing in their degrees of methylation and acetylation. Biochemical Engineering Journal 16 (2):191–201. doi:10.1016/S1369-703X(03)00037-8.
   Web of Science ®Google Scholar
• Ratajczak, W., A. Rył, A. Mizerski, K. Walczakiewicz, O. Sipak, and M. Laszczyńska. 2019. Immunomodulatory potential of gut microbiome-derived short-chain fatty acids (SCFAs). Acta Biochimica Polonica 66 (1):1–12. doi:10.18388/abp.2018_2648.
   PubMed Web of Science ®Google Scholar
• Reddy, B. S., K. Watanabe, and A. Sheinfil. 1980. Effect of dietary wheat bran, alfalfa, pectin and carrageenan on plasma cholesterol and fecal bile acid and neutral sterol excretion in rats. The Journal of Nutrition 110 (6):1247–54. doi:10.1093/jn/110.6.1247.
   PubMed Web of Science ®Google Scholar
• Ren, T., F. R. Liu, D. X. Wang, B. Li, P. Jiang, J. M. Li, H. Li, C. B. Chen, W. Wu, and L. L. Jiao. 2023. Rhamnogalacturonan-I enriched pectin from steamed ginseng ameliorates lipid metabolism in type 2 diabetic rats via gut microbiota and AMPK pathway. Journal of Ethnopharmacology 301(January): 115862. doi:10.1016/j.jep.2022.115862.
   PubMed Web of Science ®Google Scholar
• Rossen, N. G., J. K. MacDonald, E. M. de Vries, G. R. D’Haens, W. M. de Vos, E. G. Zoetendal, and C. Y. Ponsioen. 2015. Fecal microbiota transplantation as novel therapy in gastroenterology: A systematic review. World Journal of Gastroenterology 21 (17):5359–71. doi:10.3748/wjg.v21.i17.5359.
   PubMed Web of Science ®Google Scholar
• Sabater, C., J. A. Molina-Tijeras, T. Vezza, N. Corzo, A. Montilla, and P. Utrilla. 2019. Intestinal anti-inflammatory effects of artichoke pectin and modified pectin fractions in the dextran sulfate sodium model of mice colitis. Artificial neural network modelling of inflammatory markers. Food & Function 10 (12):7793–805. doi:10.1039/c9fo02221j.
   PubMed Web of Science ®Google Scholar
• Sahasrabudhe, N. M., M. Beukema, L. Tian, B. Troost, J. Scholte, E. Bruininx, G. Bruggeman, M. van den Berg, A. Scheurink, H. A. Schols, et al. 2018. Dietary fiber pectin directly blocks toll-like receptor 2-1 and prevents doxorubicin-induced ileitis. Frontiers in Immunology 9 (MAR):383. doi:10.3389/fimmu.2018.00383.
   PubMedGoogle Scholar
• Salyers, A. A., S. E. West, J. R. Vercellotti, and T. D. Wilkins. 1977. Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. Applied and Environmental Microbiology 34 (5):529–33. doi:10.1128/aem.34.5.529-533.1977.
   PubMed Web of Science ®Google Scholar
• Schleimer, R. P., and S. Berdnikovs. 2017. Etiology of epithelial barrier dysfunction in patients with type 2 inflammatory diseases. The Journal of Allergy and Clinical Immunology 139 (6):1752–61. doi:10.1016/j.jaci.2017.04.010.
   PubMed Web of Science ®Google Scholar
• Schols, H. A., and A. G. Voragen. 1996. Complex pectins: Structure elucidation using enzymes. Progress in Biotechnology 14 (C):3–19.
   Google Scholar
• Slavin, J. 2013. Fiber and prebiotics: Mechanisms and health benefits. Nutrients 5 (4):1417–35. doi:10.3390/nu5041417.
   PubMed Web of Science ®Google Scholar
• Soliman, G. A. 2019. Dietary fiber, atherosclerosis, and cardiovascular disease. Nutrients 11 (5):1155. doi:10.3390/nu11051155.
   PubMed Web of Science ®Google Scholar
• Sousa, A. G., H. L. Nielsen, I. Armagan, J. Larsen, and S. O. Sørensen. 2015. The impact of rhamnogalacturonan-I side chain monosaccharides on the rheological properties of citrus pectin. Food Hydrocolloids. 47 (May):130–9. doi:10.1016/j.foodhyd.2015.01.013.
   Google Scholar
• Spahn, T. W., and T. Kucharzik. 2004. Modulating the intestinal immune system: The role of lymphotoxin and GALT organs. Gut 53 (3):456–65. doi:10.1136/gut.2003.023671.
   PubMed Web of Science ®Google Scholar
• Szentkuti, L., H. Riedesel, M. L. Enss, K. Gaertner, and W. Von Engelhardt. 1990. Pre-epithelial mucus layer in the colon of conventional and germ-free rats. The Histochemical Journal 22 (9):491–7. doi:10.1007/BF01007234.
   PubMedGoogle Scholar
• Tang, X., M. Beukema, M. Ferrari, M. T. C. Walvoort, B. J. De Haan, and P. De Vos. 2023. Efficacy of pectins with different degree of methyl-esterification and of blockiness on preventing gut epithelial cell barrier disruption and impact on sodium-glucose co-transporter expression under low and high glucose conditions. Food & Function 14 (13):6226–35. doi:10.1039/d3fo01436c.
   PubMed Web of Science ®Google Scholar
• Takiishi, T., C. I. M. Fenero, and N. O. S. Câmara. 2017. Intestinal barrier and gut microbiota: Shaping our immune responses throughout life. Tissue Barriers 5 (4):e1373208. doi:10.1080/21688370.2017.1373208.
   PubMed Web of Science ®Google Scholar
• Thaiss, C. A., N. Zmora, M. Levy, and E. Elinav. 2016. The microbiome and innate immunity. Nature 535 (7610):65–74. doi:10.1038/nature18847.
   PubMed Web of Science ®Google Scholar
• Thakur, B. R., R. K. Singh, and A. K. Handa. 2009. Chemistry and uses of pectin—A review. Critical Reviews in Food Science and Nutrition 37 (1):47–73. doi:10.1080/10408399709527767.
   Google Scholar
• Tian, L. M., G. Bruggeman, M. Van den Berg, K. Borewicz, A. J. W. Scheurink, E. Bruininx, P. De Vos, H. Smidt, H. A. Schols, and H. Gruppen. 2017. Effects of pectin on fermentation characteristics, carbohydrate utilization, and microbial community composition in the gastrointestinal tract of weaning pigs. Molecular Nutrition & Food Research 61 (1):1600186. doi:10.1002/mnfr.201600186.
   Web of Science ®Google Scholar
• Tian, L. M., J. Scholte, K. Borewicz, B. Van den Bogert, H. Smidt, A. J. Scheurink, H. Gruppen, and H. A. Schols. 2016. Effects of pectin supplementation on the fermentation patterns of different structural carbohydrates in rats. Molecular Nutrition & Food Research 60 (10):2256–66. doi:10.1002/mnfr.201600149.
   PubMed Web of Science ®Google Scholar
• Tolhurst, G., H. Heffron, Y. S. Lam, H. E. Parker, A. M. Habib, E. Diakogiannaki, J. Cameron, J. Grosse, F. Reimann, and F. M. Gribble. 2012. Short-chain fatty acids stimulate glucagon-like peptide-1 secretion via the G-protein-coupled receptor FFAR2. Diabetes 61 (2):364–71. doi:10.2337/db11-1019.
   PubMed Web of Science ®Google Scholar
• Turner, J. R. 2009. Intestinal mucosal barrier function in health and disease. Nature Reviews. Immunology 9 (11):799–809. doi:10.1038/nri2653.
   PubMed Web of Science ®Google Scholar
• Vincken, J. P., H. A. Schols, R. J. Oomen, M. C. McCann, P. Ulvskov, A. G. J. Voragen, and R. G. F. Visser. 2003. If homogalacturonan were a side chain of rhamnogalacturonan I. Implications for cell wall architecture. Plant Physiology 132 (4):1781–9. doi:10.1104/pp.103.022350.
   PubMed Web of Science ®Google Scholar
• Visser, J., J. Rozing, A. Sapone, K. Lammers, and A. Fasano. 2009. Tight junctions, intestinal permeability, and autoimmunity. Annals of the New York Academy of Sciences 1165 (1):195–205. doi:10.1111/j.1749-6632.2009.04037.x.
   PubMedGoogle Scholar
• Vogt, L. M., N. M. Sahasrabudhe, U. Ramasamy, D. Meyer, G. Pullens, M. M. Faas, K. Venema, H. A. Schols, and P. De Vos. 2016. The impact of lemon pectin characteristics on TLR activation and T84 intestinal epithelial cell barrier function. Journal of Functional Foods 22(April): 398–407. doi:10.1016/j.jff.2016.02.002.
   Web of Science ®Google Scholar
• Walker, A. W., and T. D. Lawley. 2013. Therapeutic modulation of intestinal dysbiosis. Pharmacological Research 69 (1):75–86. doi:10.1016/j.phrs.2012.09.008.
   PubMed Web of Science ®Google Scholar
• Wang, J. W., C. H. Kuo, F. C. Kuo, Y. K. Wang, W. H. Hsu, F. J. Yu, H. M. Hu, P. I. Hsu, J. Y. Wang, and D. C. Wu. 2019. Fecal microbiota transplantation: Review and update. Journal of the Formosan Medical Association = Taiwan Yi Zhi 118 Suppl 1(March): S23–S31. doi:10.1016/j.jfma.2018.08.011.
   PubMed Web of Science ®Google Scholar
• Wang, X., L. Wilson, and D. J. Cosgrove. 2020. Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep. Journal of Experimental Botany 71 (9):2629–40. doi:10.1093/jxb/eraa059.
   PubMed Web of Science ®Google Scholar
• Wikiera, A., M. Irla, and M. Mika. 2014. Health-promoting properties of pectin. Postepy Higieny i Medycyny Doswiadczalnej (Online) 68 (January):590–6. doi:10.5604/17322693.1102342.
   PubMedGoogle Scholar
• Willats, W. G., J. P. Knox, and J. D. Mikkelsen. 2006. Pectin: New insights into an old polymer are starting to gel. Trends in Food Science & Technology 17 (3):97–104. doi:10.1016/j.tifs.2005.10.008.
   Web of Science ®Google Scholar
• Williams, L. M., H. A. Scott, and L. G. Wood. 2019. Soluble fibre as a treatment for inflammation in asthma. Journal of Nutrition & Intermediary Metabolism 18(December): 100108. doi:10.1016/j.jnim.2019.100108.
   Google Scholar
• Wu, C., L.-L. Pan, Y. Luo, W. Niu, X. Fang, W. Liang, J. Li, H. Li, X. Pan, G. Yang, et al. 2019. Low methoxyl pectin protects against autoimmune diabetes and associated caecal dysfunction. Molecular Nutrition & Food Research 63 (21):e1900307. doi:10.1002/mnfr.201900307.
   PubMed Web of Science ®Google Scholar
• Wu, D. M., X. Q. Ye, R. J. Linhardt, X. W. Liu, K. Zhu, C. X. Yu, T. Ding, D. H. Liu, Q. J. He, and S. G. Chen. 2021. Dietary pectic substances enhance gut health by its polycomponent: A review. Comprehensive Reviews in Food Science and Food Safety 20 (2):2015–39. doi:10.1111/1541-4337.12723.
   PubMed Web of Science ®Google Scholar
• Wu, D. M., J. Q. Zheng, G. Z. Mao, W. W. Hu, X. Q. Ye, R. J. Linhardt, and S. G. Chen. 2020. Rethinking the impact of RG-I mainly from fruits and vegetables on dietary health. Critical Reviews in Food Science and Nutrition 60 (17):2938–60. doi:10.1080/10408398.2019.1672037.
   PubMed Web of Science ®Google Scholar
• Wu, W. D., L. Zhang, B. Xia, S. L. Tang, J. J. Xie, and H. F. Zhang. 2020. Modulation of pectin on mucosal innate immune function in pigs mediated by gut microbiota. Microorganisms 8 (4):535. doi:10.3390/microorganisms8040535.
   PubMed Web of Science ®Google Scholar
• Xie, J. L., R. X. Yu, J. M. Qi, G. W. Zhang, X. C. Peng, and J. M. Luo. 2020. Pectin and inulin stimulated the mucus formation at a similar level: An omics-based comparative analysis. Journal of Food Science 85 (6):1939–47. doi:10.1111/1750-3841.15163.
   PubMed Web of Science ®Google Scholar
• Yapo, B. M. 2011. Rhamnogalacturonan-I: A structurally puzzling and functionally versatile polysaccharide from plant cell walls and mucilages. Polymer Reviews 51 (4):391–413. doi:10.1080/15583724.2011.615962.
   Web of Science ®Google Scholar
• Zhao, L., F. Zhang, X. Ding, G. Wu, Y. Y. Lam, X. Wang, H. Fu, X. Xue, C. Lu, J. Ma, et al. 2018. Gut bacteria selectively promoted by dietary fibers alleviate type 2 diabetes. Science (New York, N.Y.) 359 (6380):1151–6. doi:10.1126/science.aao5774.
   PubMed Web of Science ®Google Scholar
• Zhao, Y. Y., J. F. Bi, J. Y. Yi, X. Y. Wu, Y. C. Ma, and R. P. Li. 2021. Pectin and homogalacturonan with small molecular mass modulate microbial community and generate high SCFAs via in vitro gut fermentation. Carbohydrate Polymers 269(October): 118326. doi:10.1016/j.carbpol.2021.118326.
   PubMed Web of Science ®Google Scholar