The proximity of the N- and C- termini of bovine knob domains enable engineering of target specificity into polypeptide chains

References

• Stanfield RL, Wilson IA, Smider VV, Smider VV. Conservation and diversity in the ultralong third heavy-chain complementarity-determining region of bovine antibodies. Science Immunology. 2016;1(1):1–12. doi:10.1126/sciimmunol.aaf7962. PMID: 27574710
   Web of Science ®Google Scholar
• Wang F, Ekiert DC, Ahmad I, Yu W, Zhang Y, Bazirgan O, Torkamani A, Raudsepp T, Mwangi W, Criscitiello MF, et al. Reshaping antibody diversity. Cell. 2013;153(6):1379–93. doi:10.1016/j.cell.2013.04.049. PMID: 23746848.
   PubMed Web of Science ®Google Scholar
• Stanfield RL, Berndsen ZT, Huang R, Sok D, Warner G, Torres JL, Burton DR, Ward AB, Wilson IA, Smider VV, et al. Structural basis of broad HIV neutralization by a vaccine-induced cow antibody. Sci Adv. 2020;6(22):eaba0468. doi:10.1126/sciadv.aba0468. PMID: 32518821
   PubMed Web of Science ®Google Scholar
• Macpherson A, Laabei M, Ahdash Z, Graewert MA, Birtley JR, Schulze ME, Crennell S, Robinson SA, Holmes B, Oleinikovas V, et al. The allosteric modulation of complement C5 by knob domain peptides. eLife. 2021;10:e63586. doi:10.7554/eLife.63586. PMID: 33570492.
   PubMed Web of Science ®Google Scholar
• Macpherson A, Scott-Tucker A, Spiliotopoulos A, Simpson C, Staniforth J, Hold A, Snowden J, Manning L, van den Elsen J, Lawson ADG. Isolation of antigen-specific, disulphide-rich knob domain peptides from bovine antibodies. PLoS Biology. 2020;18(9):e3000821. doi:10.1371/journal.pbio.3000821. PMID: 32886672
   PubMed Web of Science ®Google Scholar
• Dong J, Finn JA, Larsen PA, Smith TPL, Crowe JE Jr. Structural diversity of ultralong CDRH3s in seven bovine antibody heavy chains. Front Immunol. 2019;10:558. doi:10.3389/fimmu.2019.00558. PMID: 30967877.
   PubMed Web of Science ®Google Scholar
• Macpherson A, Birtley JR, Broadbridge RJ, Brady K, Schulze MED, Tang Y, Joyce C, Saunders K, Bogle G, Horton J, et al. The chemical synthesis of knob domain antibody fragments. ACS Chem Biol. 2021;16(9):1757–69. doi:10.1021/acschembio.1c00472. PMID: 34406751.
   PubMed Web of Science ®Google Scholar
• Markovsky E, Baabur-Cohen H, Eldar-Boock A, Omer L, Tiram G, Ferber S, Ofek P, Polyak D, Scomparin A, Satchi-Fainaro R. Administration, distribution, metabolism and elimination of polymer therapeutics. Journal of Controlled Release: Official Journal of the Controlled Release Society. 2012;161(2):446–60. doi:10.1016/j.jconrel.2011.12.021. PMID: 22286005
   PubMed Web of Science ®Google Scholar
• Peters T. 2 - The albumin molecule: its structure and chemical properties. In: Peters T, editor. All About Albumin. San Diego: Academic Press; 1995. p. 9–II.
   Google Scholar
• Chaudhury C, Mehnaz S, Robinson JM, Hayton WL, Pearl DK, Roopenian DC, Anderson CL. The major histocompatibility complex-related Fc receptor for IgG (FcRn) binds albumin and prolongs its lifespan. J Exp Med. 2003;197(3):315–22. doi:10.1084/jem.20021829. PMID: 12566415
   PubMed Web of Science ®Google Scholar
• Waldmann TA, Strober W. Metabolism of immunoglobulins. Prog Allergy. 1969;13:1–110. doi:10.1159/000385919. PMID: 4186070.
   PubMedGoogle Scholar
• Colvin RA, Tanwandee T, Piratvisuth T, Thongsawat S, Hui AJ, Zhang H, Ren H, Chen P-J, Chuang W-L, Sobhonslidsuk A, et al. Randomized, controlled pharmacokinetic and pharmacodynamic evaluation of albinterferon in patients with chronic hepatitis B infection. Journal of Gastroenterology and Hepatology. 2015;30(1):184–91. doi:10.1111/jgh.12671. PMID: 24995515.
   PubMed Web of Science ®Google Scholar
• Lau J, Bloch P, Schaffer L, Pettersson I, Spetzler J, Kofoed J, Madsen K, Knudsen LB, McGuire J, Steensgaard DB, et al. Discovery of the once-weekly glucagon-like peptide-1 (GLP-1) analogue semaglutide. J Med Chem. 2015;58(18):7370–80. doi:10.1021/acs.jmedchem.5b00726. PMID: 26308095.
   PubMed Web of Science ®Google Scholar
• Lewis AL, McEntee N, Holland J, Patel A. Development and approval of rybelsus (oral semaglutide): ushering in a new era in peptide delivery. Drug Deliv Transl Res. 2021;12(1):1–6. doi:10.1007/s13346-021-01000-w. PMID: 34024013
   PubMed Web of Science ®Google Scholar
• Huang X, Wang C, Ma T, Huang Z, Zhou H, Xu L, Zhang R, Zhao J, Zhang Y, Huang Z, et al. The efficacy of combined cisplatin and nanoparticle albumin-bound paclitaxel in a stage iv pancreatic squamous cell carcinoma patient with a somatic BRCA2 mutation: a case report. Front Oncol. 2021;11:585983. doi:10.3389/fonc.2021.585983. PMID: 33996534.
   PubMed Web of Science ®Google Scholar
• Nilsen J, Trabjerg E, Grevys A, Azevedo C, Brennan SO, Stensland M, Wilson J, Sand KMK, Bern M, Dalhus B, et al. An intact C-terminal end of albumin is required for its long half-life in humans. Communications Biology. 2020;3(1):181. doi:10.1038/s42003-020-0903-7. PMID: 32313072.
   PubMed Web of Science ®Google Scholar
• Labrijn AF, Janmaat ML, Reichert JM, Parren P. Bispecific antibodies: a mechanistic review of the pipeline. Nat Rev Drug Discov. 2019;18(8):585–608. doi:10.1038/s41573-019-0028-1. PMID: 31175342
   PubMed Web of Science ®Google Scholar
• Xu Y, Lee J, Tran C, Heibeck TH, Wang WD, Yang J, Stafford RL, Steiner AR, Sato AK, Hallam TJ, et al. Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system. mAbs. 2015;7(1):231–42. doi:10.4161/19420862.2015.989013. PMID: 25427258.
   PubMed Web of Science ®Google Scholar
• Labrijn AF, Meesters JI, de Goeij BECG, van den Bremer ETJ, Neijssen J, van Kampen MD, Strumane K, Verploegen S, Kundu A, Gramer MJ, et al. Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange. Nat Protoc. 2013;110(13):5145–50. doi:10.1073/pnas.1220145110. PMID: 23479652.
   Google Scholar
• Jensen RK, Pihl R, Gadeberg TAF, Jensen JK, Andersen KR, Thiel S, Laursen NS, Andersen GR. A potent complement factor C3-specific nanobody inhibiting multiple functions in the alternative pathway of human and murine complement. The Journal of Biological Chemistry. 2018;293(17):6269–81. doi:10.1074/jbc.RA117.001179. PMID: 29497000
   PubMed Web of Science ®Google Scholar
• Oganesyan V, Damschroder MM, Cook KE, Li Q, Gao C, Wu H, Dall’Acqua WF. Structural insights into neonatal Fc receptor-based recycling mechanisms. The Journal of Biological Chemistry. 2014;289(11):7812–24. doi:10.1074/jbc.M113.537563. PMID: 24469444
   PubMed Web of Science ®Google Scholar
• Sand KM, Dalhus B, Christianson GJ, Bern M, Foss S, Cameron J, Sleep D, Bjørås M, Roopenian DC, Sandlie I, et al. Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies. The Journal of Biological Chemistry. 2014;289(24):17228–39. doi:10.1074/jbc.M113.522565. PMID: 24764301.
   PubMed Web of Science ®Google Scholar
• Andersen JT, Dalhus B, Cameron J, Daba MB, Plumridge A, Evans L, Brennan SO, Gunnarsen KS, Bjørås M, Sleep D, et al. Structure-based mutagenesis reveals the albumin-binding site of the neonatal Fc receptor. Nat Commun. 2012;3(1):610. doi:10.1038/ncomms1607. PMID: 22215085.
   PubMedGoogle Scholar
• Andersen JT, Daba MB, Berntzen G, Michaelsen TE, Sandlie I. Cross-species binding analyses of mouse and human neonatal Fc receptor show dramatic differences in immunoglobulin G and albumin binding. The Journal of Biological Chemistry. 2010;285(7):4826–36. doi:10.1074/jbc.M109.081828. PMID: 20018855
   PubMed Web of Science ®Google Scholar
• Berens SJ, Wylie DE, Lopez OJ. Use of a single VH family and long CDR3s in the variable region of cattle Ig heavy chains. Int Immunol. 1997;9(1):189–99. doi:10.1093/intimm/9.1.189. PMID: 9043960
   PubMed Web of Science ®Google Scholar
• Mitchell LS, Colwell LJ. Comparative analysis of nanobody sequence and structure data. Proteins. 2018;86(7):697–706. doi:10.1002/prot.25497. PMID: 29569425
   PubMed Web of Science ®Google Scholar
• Micsonai A, Wien F, Bulyaki E, Kun J, Moussong E, Lee YH, Goto Y, Refregiers M, Kardos J. BeStSel: a web server for accurate protein secondary structure prediction and fold recognition from the circular dichroism spectra. Nucleic Acids Res. 2018;46(W1):315–22. doi:10.1093/nar/gky497. PMID: 29893907
   PubMed Web of Science ®Google Scholar
• Krah S, Schröter C, Eller C, Rhiel L, Rasche N, Beck J, Sellmann C, Günther R, Toleikis L, Hock B, et al. Generation of human bispecific common light chain antibodies by combining animal immunization and yeast display. Protein Engineering, Design and Selection. 2017;30:291–301. doi:10.1093/protein/gzw077. PMID: 28062646.
   PubMed Web of Science ®Google Scholar
• Macpherson A, Liu X, Dedi N, Kennedy J, Carrington B, Durrant O, Heywood S, van den Elsen J, Lawson ADG. The rational design of affinity-attenuated OmCI for the purification of complement C5. The Journal of Biological Chemistry. 2018;293(36):14112–21. doi:10.1074/jbc.RA118.004043. PMID: 30030376
   PubMed Web of Science ®Google Scholar