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DNA Dynamics and Chromosome Structure

A RAG1 and RAG2 Tetramer Complex Is Active in Cleavage in V(D)J Recombination

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Pages 4664-4671 | Received 29 Jan 1999, Accepted 01 Apr 1999, Published online: 28 Mar 2023

REFERENCES

  • Akamatsu, Y., and J. Oettinger 1998. Distinct roles of RAG1 and RAG2 in binding the V(D)J recombination signal sequences. Mol. Cell. Biol. 18:4670–4678.
  • Besmer, E., J. Mansilla-Soto, S. Cassard, D. J. Sawchuk, G. Brown, M. Sadofsky, S. M. Lewis, M. C. Nussenzweig, and J. Cortes 1998. Hairpin coding end opening is mediated by RAG1 and RAG2 proteins. Mol. Cell 2:817–828.
  • Bullock, B. P., and J. Habener 1998. Phosphorylation of the cAMP response element binding protein CREB by cAMP-dependent protein kinase A and glycogen synthase kinase-3 alters DNA-binding affinity, conformation, and increases net charge. Biochemistry 37:3795–3809.
  • Difilippantonio, M. J., C. J. McMahan, Q. M. Eastman, E. Spanopoulou, and J. Schatz 1996. RAG1 mediates signal sequence recognition and recruitment of RAG2 in V(D)J recombination. Cell 87:253–262.
  • Eastman, Q. M., T. M. J. Leu, and J. Schatz 1996. Initiation of V(D)J recombination in-vitro obeying the 12/23-rule. Nature 380:85–88.
  • Gellert, M. 1997. Recent advances in understanding V(D)J recombination. Adv. Immunol. 64:39–64.
  • Hiom, K., and J. Gellert 1998. Assembly of a 12/23 paired signal complex: a critical control point in V(D)J recombination. Mol. Cell 1:1011–1019.
  • Hiom, K., and J. Gellert 1997. A stable RAG1-RAG2-DNA complex that is active in V(D)J cleavage. Cell 88:65–72.
  • Kim, D. R., and J. Oettinger 1998. Functional analysis of coordinated cleavage in V(D)J recombination. Mol. Cell. Biol. 18:4679–4688.
  • Lavoie, B. D., and J. Chaconas 1996. Transposition of phage Mu DNA. Curr. Top. Microbiol. Immunol. 204:83–102.
  • Lewis, S. M. 1994. The mechanism of V(D)J joining: lessons from molecular, immunological, and comparative analyses. Adv. Immunol. 56:27–150.
  • May, G., C. Sutton, and J. Gould 1991. Purification and characterization of Ku-2, an octamer-binding protein related to the autoantigen Ku. J. Biol. Chem. 266:3052–3059.
  • McBlane, J. F., D. C. van Gent, D. A. Ramsden, C. Romeo, C. A. Cuomo, M. Gellert, and J. Oettinger 1995. Cleavage at a V(D)J recombination signal requires only RAG1 and RAG2 proteins and occurs in two steps. Cell 83:387–395.
  • McMahan, C. J., M. J. Sadofsky, and J. Schatz 1997. Definition of a large region of RAG1 that is important for coimmunoprecipitation of RAG2. J. Immunol. 158:2202–2210.
  • Mo, X., B. Tu, and J. Sadofsky 1999. RAG1 and RAG2 cooperate in specific binding to the recombination signal sequence in vitro. J. Biol. Chem. 274:7025–7032.
  • Oettinger, M. A., D. G. Schatz, C. Gorka, and J. Baltimore 1990. RAG-1 and RAG-2, adjacent genes that synergistically activate V(D)J recombination. Science 248:1517–1523.
  • Orchard, K., and J. May 1993. An EMSA-based method for determining the molecular weight of a protein-DNA complex. Nucleic Acids Res. 21:3335–3336.
  • Rodgers, K. K., Z. Bu, K. G. Fleming, D. G. Schatz, D. M. Engelman, and J. Coleman 1996. A zinc-binding domain involved in the dimerization of RAG1. J. Mol. Biol. 260:70–84.
  • Sadofsky, M. J., J. E. Hesse, J. F. McBlane, and J. Gellert 1993. Expression and V(D)J recombination activity of mutated RAG-1 proteins. Nucleic Acids Res. 21:5644–5650.
  • Sadofsky, M. J., J. E. Hesse, D. C. van Gent, and J. Gellert 1995. RAG-1 mutations that affect the target specificity of V(D)J recombination: a possible direct role of RAG-1 in site recognition. Genes Dev. 9:2193–2199.
  • Sawchuk, D. J., F. Weis-Garcia, S. Malik, E. Besmer, M. Bustin, M. C. Nussenzweig, and J. Cortes 1997. V(D)J recombination: modulation of RAG1 and RAG2 cleavage activity on 12/23 substrates by whole cell extract and DNA-bending proteins. J. Exp. Med. 185:2025–2032.
  • Schatz, D. G., M. A. Oettinger, and J. Baltimore 1989. The V(D)J recombination activating gene, RAG-1. Cell 59:1035–1048.
  • Spanopoulou, E., F. Zaitseva, F. Wang, S. Santagata, D. Baltimore, and J. Panayotou 1996. The homeodomain region of Rag-1 reveals the parallel mechanisms of bacterial and V(D)J recombination. Cell 87:263–276.
  • Steen, S. B., L. Gomelsky, S. L. Speidel, and J. Roth 1997. Initiation of V(D)J recombination in vivo: role of recombination signal sequences in formation of single and paired double-strand breaks. EMBO J. 16:2656–2664.
  • Swanson, P. C., and J. Desiderio 1998. V(D)J recombination signal recognition: distinct, overlapping DNA-protein contacts in complexes containing RAG1 with and without RAG2. Immunity 9:115–125.
  • Tonegawa, S. 1983. Somatic generation of antibody diversity. Nature 302:575–581.
  • van Gent, D. C., K. Hiom, T. T. Paull, and J. Gellert 1997. Stimulation of V(D)J cleavage by high mobility group proteins. EMBO J. 16:2665–2670.
  • van Gent, D. C., J. F. McBlane, D. A. Ramsden, M. J. Sadofsky, J. E. Hesse, and J. Gellert 1995. Initiation of V(D)J recombination in a cell-free system. Cell 81:925–934.
  • van Gent, D. C., K. Mizuuchi, and J. Gellert 1996. Similarities between initiation of V(D)J recombination and retroviral integration. Science 271:1592–1594.
  • van Gent, D. C., D. A. Ramsden, and J. Gellert 1996. The Rag1 and Rag2 proteins establish the 12/23-rule in V(D)J recombination. Cell 85:107–113.
  • West, R. B., and J. Lieber 1998. The RAG-HMG1 complex enforces the 12/23 rule of V(D)J recombination specifically at the double-hairpin formation step. Mol. Cell. Biol. 18:6408–6415.

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