8
Views
26
CrossRef citations to date
0
Altmetric
DNA Dynamics and Chromosome Structure

Transcriptional Activity and Chromatin Structure of Enhancer-Deleted rRNA Genes in Saccharomyces cerevisiae

, &
Pages 4953-4960 | Received 19 Oct 1998, Accepted 30 Mar 1999, Published online: 28 Mar 2023

REFERENCES

  • Banditt, M. 1998. Ph.D. thesis no. 12874. Eidgenössische Technische Hochschule Zürich, Zürich, Switzerland.
  • Becker, D. M., and J. Guarente 1991. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194:182–187.
  • Brewer, B. J., and J. Fangman 1988. A replication fork barrier at the 3′ end of yeast ribosomal RNA genes. Cell 55:637–643.
  • Butlin, M., and J. Quincey 1991. The yeast rRNA gene enhancer does not function by recycling RNA polymerase I and cannot act as a UAS. Curr. Genet. 20:9–16.
  • Conconi, A., R. M. Widmer, T. Koller, and J. Sogo 1989. Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle. Cell 57:753–761.
  • Dammann, R., R. Lucchini, T. Koller, and J. Sogo 1993. Chromatin structures and transcription of rDNA in yeast Saccharomyces cerevisiae. Nucleic Acids Res. 21:2331–2338.
  • Dammann, R., R. Lucchini, T. Koller, and J. Sogo 1995. Transcription in the yeast rDNA locus: distribution of the active gene copies and chromatin structure of their flanking regulatory sequences. Mol. Cell. Biol. 15:5294–5303.
  • Elion, E. A., and J. Warner 1984. The major promoter element of rRNA transcription in yeast lies 2 kb upstream. Cell 39:663–673.
  • Elion, E. A., and J. Warner 1986. An RNA polymerase I enhancer in Saccharomyces cerevisiae. Mol. Cell. Biol. 6:2089–2097.
  • Hanson, C. V., K. J. Shen, and J. Hearst 1976. Cross-linking of DNA in situ as a probe for chromatin structure. Science 193:62–64.
  • Johnson, S. P., and J. Warner 1989. Unusual enhancer function in yeast rRNA transcription. Mol. Cell. Biol. 9:4986–4993.
  • Kato, H., M. Nagamine, R. Kominami, and J. Muramatsu 1986. Formation of the transcription initiation complex on mammalian rDNA. Mol. Cell. Biol. 6:3418–3427.
  • Kief, D. R., and J. Warner 1981. Coordinated control of synthesis of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae. Mol. Cell. Biol. 1:1007–1015.
  • Kormanec, J., and J. Farkasovsky 1994. Isolation of total RNA from yeast and bacteria and detection of rRNA in Northern blots. BioTechniques 17:838–842.
  • Kulkens, T., C. A. F. M. van der Sande, A. F. Dekker, H. van Heerikhuizen, and J. Planta 1992. A system to study transcription by yeast RNA polymerase I within the chromosomal context: functional analysis of the ribosomal DNA enhancer and the RBP1/REB1 binding sites. EMBO J. 11:4665–4674.
  • Lang, W. H., and J. Reeder 1993. The REB1 site is an essential component of a terminator for RNA polymerase I in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:649–658.
  • Ling, M., F. Merante, and J. Robinson 1995. A rapid and reliable DNA preparation method for screening a large number of yeast clones by polymerase chain reaction. Nucleic Acids Res. 23:4924–4925.
  • Long, E. O., and J. Dawid 1980. Repeated genes in eucaryotes. Annu. Rev. Biochem. 49:727–764.
  • Lucchini, R., and J. Sogo 1992. Different chromatin structures along the spacers flanking active and inactive Xenopus rRNA genes. Mol. Cell. Biol. 12:4288–4296.
  • Lucchini, R., and J. Sogo 1994. Chromatin structure and transcriptional activity around the replication forks arrested at the 3′ end of the yeast rRNA genes. Mol. Cell. Biol. 14:318–326.
  • Lucchini, R., and J. Sogo 1995. Replication of transcriptionally active chromatin. Nature 374:276–280.
  • Morrow, B. E., S. P. Johnson, and J. Warner 1993. The rDNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 13:1283–1289.
  • Morrow, B. E., Q. Ju, and J. Warner 1990. Purification and characterization of the yeast rDNA binding protein REB1. J. Biol. Chem. 265:20778–20783.
  • Musters, W., J. Knol, P. Maas, A. F. Dekker, H. van Heerikhuizen, and J. Planta 1989. Linker scanning of the yeast RNA polymerase I promoter. Nucleic Acids Res. 17:9661–9678.
  • Osheim, Y. N., E. B. Mougey, J. Windle, M. Anderson, M. O’Reilly, O. L. Miller Jr., A. Beyer, and J. Sollner-Webb 1996. Metazoan rDNA enhancer acts by making more genes transcriptionally active. J. Cell Biol. 133:943–954.
  • Philippsen, P., M. Thomas, R. A. Kramer, and J. Davis 1978. Unique arrangement of coding sequences for 5S, 5.8S, 18S and 25S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loops and hybridisation analysis. J. Mol. Biol. 123:387–404.
  • Reeder, R. H. 1989. Regulatory elements of the generic ribosomal gene. Curr. Opin. Cell Biol. 1:466–474.
  • Sambrook, J., E. F. Fritsch, T. Maniatis 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
  • Schultz, M. C., S. Y. Choe, and J. Reeder 1993. In vitro definition of the yeast RNA polymerase I enhancer. Mol. Cell. Biol. 13:2644–2654.
  • Sigurdson, C. D., M. E. Gaarder, and J. Livingston 1981. Characterization of the transmission during cytoductant formation of the 2μm DNA plasmid from Saccharomyces. Mol. Gen. Genet. 183:59–65.
  • Sikorski, R. S., and J. Hieter 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19–27.
  • Skryabin, K. G., M. A. Eldarov, V. L. Larionov, A. A. Bayev, J. Klootwijk, V. C. H. F. de Regt, G. M. Veldman, R. Planta, O. I. Georgiev, and J. Hadjiolov 1984. Structure and function of the nontranscribed spacer region of yeast rDNA. Nucleic Acids Res. 12:2955–2968.
  • Sogo, J. M., P. J. Ness, R. M. Widmer, R. W. Parish, and J. Koller 1984. Psoralen crosslinking as a probe for the structure of active nucleolar chromatin. J. Mol. Biol. 178:897–928.
  • van der Sande, C. A. F. M., T. Kulkens, A. B. Kramer, I. J. de Wijs, H. van Heerikhuizen, J. Klootwijk, and J. Planta 1989. Termination of transcription by yeast RNA polymerase I. Nucleic Acids Res. 17:9127–9146.
  • Venema, J., and J. Tollervey 1995. Processing of pre-ribosomal RNA in Saccharomyces cerevisiae. Yeast 11:1629–1650.
  • Warner, J. R. 1989. Synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol. Rev. 53:256–271.
  • Wu, J.-R., and J. Gilbert 1995. Rapid DNA preparation for 2D gel analysis of replication intermediates. Nucleic Acids Res. 23:3997–3998.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.