RanGTP-Regulated Interactions of CRM1 with Nucleoporins and a Shuttling DEAD-Box Helicase

REFERENCES

• Arts, G.-J., L. Englmeier, and J. Mattaj 1997. Energy- and temperature-dependent in vitro export of RNA from synthetic nuclei. Biol. Chem. 378:641–649.
   PubMedGoogle Scholar
• Arts, G.-J., M. Fornerod, and J. Mattaj 1998. Identification of a nuclear export receptor for tRNA. Curr. Biol. 8:305–314.
   PubMed Web of Science ®Google Scholar
• Askjaer, P., T. H. Jensen, J. Nilsson, L. Englmeier, and J. Kjems 1998. The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP. J. Biol. Chem. 273:33414–33422.
   PubMedGoogle Scholar
• Bastos, R., L. Ribas de Pouplana, M. Enarson, K. Bodoor, and J. Burke 1997. Nup84, a novel nucleoporin that is associated with CAN/Nup214 on the cytoplasmic face of the nuclear pore complex. J. Cell Biol. 137:989–1000.
   PubMedGoogle Scholar
• Belgareh, N., C. Snay-Hodge, F. Pasteau, S. Dagher, C. N. Cole, and J. Doye 1998. Functional characterization of a Nup159p-containing nuclear pore subcomplex. Mol. Biol. Cell 9:3475–3492.
   PubMedGoogle Scholar
• Bischoff, F. R., and J. Görlich 1997. RanBP1 is crucial for the release of RanGTP from importin β-related nuclear transport factors. FEBS Lett. 419:249–254.
   PubMed Web of Science ®Google Scholar
• Bischoff, F. R., and J. Ponstingl 1995. Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Methods Enzymol. 257:135–144.
   PubMedGoogle Scholar
• Bodendorf, U., and N. Salome. Unpublished results.
   Google Scholar
• Bogerd, H. P., A. Echarri, T. M. Ross, and J. Cullen 1998. Inhibition of human immunodeficiency virus Rev and human T-cell leukemia virus Rex function, but not Mason-Pfizer monkey virus constitutive transport element activity, by a mutant human nucleoporin targeted to Crm1. J. Virol. 72:8627–8635.
   PubMedGoogle Scholar
• Bogerd, H. P., R. A. Fridell, R. E. Benson, J. Hua, and J. Cullen 1996. Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay. Mol. Cell. Biol. 16:4207–4214.
   PubMedGoogle Scholar
• Chuang, R. Y., P. L. Weaver, Z. Liu, and J. Chang 1997. Requirement of the DEAD-box protein ded1p for messenger RNA translation. Science 275:1468–1471.
   PubMed Web of Science ®Google Scholar
• Cotmore, S. F., and J. Tattersall 1990. Alternate splicing in a parvoviral nonstructural gene links a common amino-terminal sequence to downstream domains which confer radically different localization and turnover characteristics. Virology 177:477–487.
   PubMedGoogle Scholar
• Dahlberg, J. E., and J. Lund 1998. Functions of the GTPase Ran in RNA export from the nucleus. Curr. Opin. Cell Biol. 10:400–408.
   PubMedGoogle Scholar
• Englmeier, L., J. C. Olivo, and J. Mattaj 1999. Receptor-mediated substrate translocation through the nuclear pore complex without nucleotide triphosphate hydrolysis. Curr. Biol. 9:30–41.
   PubMedGoogle Scholar
• Finlay, D. R., D. D. Newmeyer, T. M. Price, and J. Forbes 1987. Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores. J. Cell Biol. 104:189–200.
   PubMed Web of Science ®Google Scholar
• Fischer, U., J. Huber, W. C. Boelens, I. W. Mattaj, and J. Lührmann 1995. The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. Cell 82:475–483.
   PubMed Web of Science ®Google Scholar
• Floer, M., G. Blobel, and J. Rexach 1997. Disassembly of RanGTP-karyopherin β complex, an intermediate in nuclear protein import. J. Biol. Chem. 272:19538–19546.
   PubMedGoogle Scholar
• Fornerod, M., J. Boer, S. van Baal, H. Morreau, and J. Grosveld 1996. Interaction of cellular proteins with the leukemia specific fusion proteins DEK-CAN and SET-CAN and their normal counterpart, the nucleoporin CAN. Oncogene 13:1801–1808.
   PubMedGoogle Scholar
• Fornerod, M., M. Ohno, M. Yoshida, and J. Mattaj 1997. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 90:1051–1060.
   PubMed Web of Science ®Google Scholar
• Fornerod, M., J. van Deursen, S. van Baal, A. Reynolds, D. Davis, K. G. Murti, J. Fransen, and J. Grosveld 1997. The human homologue of yeast CRM1 is in a dynamic subcomplex with CAN/Nup214 and a novel nuclear pore component Nup88. EMBO J. 16:807–816.
   PubMedGoogle Scholar
• Fukuda, M., S. Asano, T. Nakamura, M. Adachi, M. Yoshida, M. Yanagida, and J. Nishida 1997. CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature 390:308–311.
   PubMed Web of Science ®Google Scholar
• Görlich, D., M. Dabrowski, F. R. Bischoff, U. Kutay, P. Bork, E. Hartmann, S. Prehn, and J. Izaurralde 1997. A novel class of RanGTP binding proteins. J. Cell Biol. 138:65–80.
   PubMed Web of Science ®Google Scholar
• Görlich, D., N. Panté, U. Kutay, U. Aebi, and J. Bischoff 1996. Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15:5584–5594.
   PubMed Web of Science ®Google Scholar
• Gururajan, R., O. K. H. Perry, D. A. Melton, and J. Weeks 1991. The Xenopus localized messenger RNA An3 may encode an ATP-dependent RNA helicase. Nature 349:717–719.
   PubMedGoogle Scholar
• Gururajan, R., and J. Weeks 1997. An3 protein encoded by a localized maternal mRNA in Xenopus laevis is an ATPase with substrate-specific RNA helicase activity. Biochim. Biophys. Acta 1350:169–182.
   PubMedGoogle Scholar
• Hanover, J. A., C. K. Cohen, M. C. Willingham, and J. Park 1987. O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins. J. Biol. Chem. 262:9887–9894.
   PubMedGoogle Scholar
• Hausen, P., M. Riebesell 1991. The early development of Xenopus laevis. Springer-Verlag, Berlin, Germany.
   Google Scholar
• Holt, G. D., C. M. Snow, A. Senior, R. S. Haltiwanger, L. Gerace, and J. Hart 1987. Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine. J. Cell Biol. 104:1157–1164.
   PubMed Web of Science ®Google Scholar
• Hurwitz, M. E., C. Strambio-de-Castillia, and J. Blobel 1998. Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc. Natl. Acad. Sci. USA 95:11241–11245.
   PubMedGoogle Scholar
• Izaurralde, E., and J. Adam 1998. Transport of macromolecules between the nucleus and the cytoplasm. RNA 4:351–364.
   PubMed Web of Science ®Google Scholar
• Izaurralde, E., U. Kutay, C. von Kobbe, I. W. Mattaj, and J. Görlich 1997. The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J. 16:6535–6547.
   PubMedGoogle Scholar
• Jamieson, D. J., and J. Beggs 1991. A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP-dependent RNA helicase. Mol. Microbiol. 5:805–812.
   PubMedGoogle Scholar
• Jamieson, D. J., B. Rahe, J. Pringle, and J. Beggs 1991. A suppressor of a yeast splicing mutation (prp8-1) encodes a putative ATP-dependent RNA helicase. Nature 349:715–717.
   PubMed Web of Science ®Google Scholar
• Jarmolowski, A., W. C. Boelens, E. Izaurralde, and J. Mattaj 1994. Nuclear export of different classes of RNA is mediated by specific factors. J. Cell Biol. 124:627–635.
   PubMed Web of Science ®Google Scholar
• Jensen, T. H., A. Jensen, and J. Kjems 1995. Tools for the production and purification of full-length, N- or C-terminal 32P-labeled protein, applied to HIV-1 Gag and Rev. Gene 162:235–237.
   PubMedGoogle Scholar
• Kaffman, A., N. M. Rank, E. M. O’Neill, L. S. Huang, and J. O’Shea 1998. The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396:482–486.
   PubMed Web of Science ®Google Scholar
• Kambach, C., and J. Mattaj 1992. Intracellular distribution of the U1A protein depends on active transport and nuclear binding to U1 snRNA. J. Cell Biol. 118:11–21.
   PubMedGoogle Scholar
• Kim, F. J., A. A. Beeche, J. J. Hunter, D. J. Chin, and J. Hope 1996. Characterization of the nuclear export signal of human T-cell lymphotropic virus type 1 Rex reveals that nuclear export is mediated by position-variable hydrophobic interactions. Mol. Cell. Biol. 16:5147–5155.
   PubMedGoogle Scholar
• Kjems, J., and P. Askjaer. The Rev protein and its cellular partners. In K. T. Jeang (ed.), HIV: molecular mechanisms and clinical applications, in press. Academic Press, San Diego, Calif.
   Google Scholar
• Klebe, C., F. R. Bischoff, H. Ponstingl, and J. Wittinghofer 1995. Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1. Biochemistry 34:639–647.
   PubMedGoogle Scholar
• Kose, S., N. Imamoto, T. Tachibana, T. Shimamoto, and J. Yoneda 1997. Ran-unassisted nuclear migration of a 97-kDa component of nuclear pore-targeting complex. J. Cell Biol. 139:841–849.
   PubMedGoogle Scholar
• Kraemer, D., R. W. Wozniak, G. Blobel, and J. Radu 1994. The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm. Proc. Natl. Acad. Sci. USA 91:1519–1523.
   PubMedGoogle Scholar
• Kutay, U., F. R. Bischoff, S. Kostka, R. Kraft, and J. Görlich 1997. Export of importin α from the nucleus is mediated by a specific nuclear transport factor. Cell 90:1061–1071.
   PubMedGoogle Scholar
• Kutay, U., G. Lipowsky, E. Izaurralde, F. R. Bischoff, P. Schwarzmaier, E. Hartmann, and J. Görlich 1998. Identification of a tRNA-specific nuclear export receptor. Mol. Cell 1:359–369.
   PubMed Web of Science ®Google Scholar
• Lahn, B. T., and J. Page 1997. Functional coherence of the human Y chromosome. Science 278:675–680.
   PubMedGoogle Scholar
• Leroy, P., P. Alzari, D. Sassoon, D. Wolgemuth, and J. Fellous 1989. The protein encoded by a murine male germ cell-specific transcript is a putative ATP-dependent RNA helicase. Cell 57:549–559.
   PubMedGoogle Scholar
• Longo, F. J., L. Mathews, R. Gururajan, J. Chen, and J. Weeks 1996. Changes in nuclear localization of An3, a RNA helicase, during oogenesis and embryogenesis in Xenopus laevis. Mol. Reprod. Dev. 45:491–502.
   PubMedGoogle Scholar
• Macaulay, C., E. Meier, and J. Forbes 1995. Differential mitotic phosphorylation of proteins of the nuclear pore complex. J. Biol. Chem. 270:254–262.
   PubMed Web of Science ®Google Scholar
• Mahajan, R., C. Delphin, T. Guan, L. Gerace, and J. Melchior 1997. A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88:97–107.
   PubMed Web of Science ®Google Scholar
• Malim, M. H., and J. Cullen 1991. HIV-1 structural gene expression requires the binding of multiple Rev monomers to the viral RRE: implications for HIV-1 latency. Cell 65:241–248.
   PubMed Web of Science ®Google Scholar
• Mattaj, I. W., and J. Englmeier 1998. Nucleocytoplasmic transport: the soluble phase. Annu. Rev. Biochem. 67:265–306.
   PubMed Web of Science ®Google Scholar
• Matunis, M. J., J. Wu, and J. Blobel 1998. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex. J. Cell Biol. 140:499–509.
   PubMed Web of Science ®Google Scholar
• Meier, E., B. R. Miller, and J. Forbes 1995. Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation. J. Cell Biol. 129:1459–1472.
   PubMedGoogle Scholar
• Naeger, L. K., J. Cater, and J. Pintel 1990. The small nonstructural protein (NS2) of the parvovirus minute virus of mice is required for efficient DNA replication and infectious virus production in a cell-type-specific manner. J. Virol. 64:6166–6175.
   PubMedGoogle Scholar
• Nakielny, S., and J. Dreyfuss 1998. Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis. Curr. Biol. 8:89–95.
   PubMedGoogle Scholar
• Neuman de Vegvar, H. E., and J. Dahlberg 1990. Nucleocytoplasmic transport and processing of small nuclear RNA precursors. Mol. Cell. Biol. 10:3365–3375.
   PubMedGoogle Scholar
• Neville, M., F. Stutz, L. Lee, L. I. Davis, and J. Rosbash 1997. The importin-β family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export. Curr. Biol. 7:767–775.
   PubMed Web of Science ®Google Scholar
• Ogniewski, V., and D. L. Weeks. Unpublished results.
   Google Scholar
• Ohno, M., M. Fornerod, and J. Mattaj 1998. Nucleocytoplasmic transport: the last 200 nanometers. Cell 92:327–336.
   PubMed Web of Science ®Google Scholar
• Panté, N., R. Bastos, I. McMorrow, B. Burke, and J. Aebi 1994. Interactions and three-dimensional localization of a group of nuclear pore complex proteins. J. Cell Biol. 126:603–617.
   PubMed Web of Science ®Google Scholar
• Panté, N., A. Jarmolowski, E. Izaurralde, U. Sauder, W. Baschong, and J. Mattaj 1997. Visualizing nuclear export of different classes of RNA by electron microscopy. RNA 3:498–513.
   PubMedGoogle Scholar
• Pasquinelli, A. E., M. A. Powers, E. Lund, D. Forbes, and J. Dahlberg 1997. Inhibition of mRNA export in vertebrate cells by nuclear export signal conjugates. Proc. Natl. Acad. Sci. USA 94:14394–14399.
   PubMed Web of Science ®Google Scholar
• Pollard, V. W., and J. Malim 1998. The HIV-1 Rev protein. Annu. Rev. Microbiol. 52:491–532.
   PubMed Web of Science ®Google Scholar
• Richards, S. A., K. M. Lounsbury, K. L. Carey, and J. Macara 1996. A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. J. Cell. Biol. 134:1157–1168.
   PubMedGoogle Scholar
• Saitoh, H., D. B. Sparrow, T. Shiomi, R. T. Pu, T. Nishimoto, T. J. Mohun, and J. Dasso 1998. Ubc9p and the conjugation of SUMO-1 to RanGAP1 and RanBP2. Curr. Biol. 8:121–124.
   PubMedGoogle Scholar
• Shah, S., S. Tugendreich, and J. Forbes 1998. Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr. J. Cell Biol. 141:31–49.
   PubMed Web of Science ®Google Scholar
• Siebel, C. W., and J. Rio 1990. Regulated splicing of the Drosophila P transposable element third intron in vitro: somatic repression. Science 248:1200–1208.
   PubMedGoogle Scholar
• Sowden, J., W. Putt, K. Morrison, R. Beddington, and J. Edwards 1995. The embryonic RNA helicase gene (ERH): a new member of the DEAD box family of RNA helicases. Biochem. J. 308:839–846.
   PubMedGoogle Scholar
• Stade, K., C. S. Ford, C. Guthrie, and J. Weis 1997. Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 90:1041–1050.
   PubMed Web of Science ®Google Scholar
• Stutz, F., E. Izaurralde, I. W. Mattaj, and J. Rosbash 1996. A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus. Mol. Cell. Biol. 16:7144–7150.
   PubMedGoogle Scholar
• Stutz, F., and J. Rosbash 1998. Nuclear RNA export. Genes Dev. 12:3303–3319.
   PubMedGoogle Scholar
• Weis, K. Personal communication.
   Google Scholar
• Wen, W., J. L. Meinkoth, R. Y. Tsien, and J. Taylor 1995. Identification of a signal for rapid export of proteins from the nucleus. Cell 82:463–473.
   PubMed Web of Science ®Google Scholar
• Wickberg, A., and A. Uv. Personal communication.
   Google Scholar
• Wilm, M., L. Neubauer, A. Taylor, A. Shevchenko, and A. Bachi. De novo sequencing of proteins using the differential scanning technique. Submitted for publication.
   Google Scholar
• Wilm, M., A. Shevchenko, T. Houthaeve, S. Breit, L. Schweigerer, T. Fotsis, and J. Mann 1996. Femtomole sequencing of proteins from polyacrylamide gels by nanoelectrospray mass spectrometry. Nature 379:466–469.
   PubMed Web of Science ®Google Scholar
• Wolff, B., J. J. Sanglier, and J. Wang 1997. Leptomycin B is an inhibitor of nuclear export: inhibition of nucleocytoplasmic translocation of the human immunodeficiency virus type 1 (HIV-1) Rev protein and Rev-dependent mRNA. Chem. Biol. 4:139–147.
   PubMed Web of Science ®Google Scholar
• Wu, J., M. J. Matunis, D. Kraemer, G. Blobel, and J. Coutavas 1995. Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem. 270:14209–14213.
   PubMed Web of Science ®Google Scholar
• Yokoyama, N., N. Hayashi, T. Seki, N. Panté, T. Ohba, K. Nishii, K. Kuma, T. Hayashida, T. Miyata, U. Aebi et al.. 1995. A giant nucleopore protein that binds Ran/TC4. Nature 376:184–188.
   PubMed Web of Science ®Google Scholar