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Cell Growth and Development

Profilin II Is Alternatively Spliced, Resulting in Profilin Isoforms That Are Differentially Expressed and Have Distinct Biochemical Properties

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Pages 8209-8219 | Received 09 Mar 2000, Accepted 08 Aug 2000, Published online: 28 Mar 2023

REFERENCES

  • Ahern-Djamali, S. M., Bachmann, C., Hua, P., Reddy, S. K., Kastenmeier, A. S., Walter, U., and Hoffmann, F. M.. 1999. Identification of profilin and src homology 3 domains as binding partners for Drosophila enabled. Proc. Natl. Acad. Sci. USA 96:4977–4982
  • Anton, I. M., Lu, W., Mayer, B. J., Ramesh, N., and Geha, R. S.. 1998. The Wiskott-Aldrich syndrome protein-interacting protein (WIP) binds to the adaptor protein Nck. J. Biol. Chem. 273:20992–20995
  • Aszodi, A., Chan, D., Hunziker, E., Bateman, J. F., and Fassler, R.. 1998. Collagen II is essential for the removal of the notochord and the formation of intervertebral discs. J. Cell Biol. 143:1399–1412
  • Bjorkegren, C., Rozycki, M., Schutt, C. E., Lindberg, U., and Karlsson, R.. 1993. Mutagenesis of human profilin locates its poly(L-proline)-binding site to a hydrophobic patch of aromatic amino acids. FEBS Lett. 333:123–126
  • Bjorkegren-Sjogren, C., Korenbaum, E., Nordber, P., Lindberg, U., and Karlsson, R.. 1997. Isolation and characterization of two mutants of human profilin I that do not bind poly(L-proline). FEBS Lett. 418:258–264
  • Brenner, S., and Korn, E.. 1983. On the mechanism of actin monomer-polymer subunit exchange at steady state. J. Biol. Chem. 258:5013–5020
  • Carlier, M.-F.. 1998. Control of actin dynamics. Curr. Opin. Cell Biol. 10:45–51
  • Carlsson, L., Nystrom, L. E., Sundkvist, I., Markey, F., and Lindberg, U.. 1977. Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells. J. Mol. Biol. 115:465–483
  • Cedergren-Zeppezauer, E. S., Goonesekere, N. C., Rozycki, M. D., Myslik, J. C., Dauter, Z., Lindberg, U., and Schutt, C. E.. 1994. Crystallization and structure determination of bovine profilin at 2.0 A resolution. J. Mol. Biol. 240:459–475
  • Chaudhary, A., Chen, J., Gu, Q. M., Witke, W., Kwiatkowski, D. J., and Prestwich, G. D.. 1998. Probing the phosphoinositide 4,5-bisphosphate binding site of human profilin I. Chem. Biol. 5:273–281
  • Chomczynski, P., and Sacchi, N.. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159
  • Faivre-Sarrailh, C., Lena, J. Y., Had, L., Vignes, M., and Lindberg, U.. 1993. Location of profilin at presynaptic sites in the cerebellar cortex; implication for the regulation of the actin polymerization state during axonal elongation and synaptogenesis. J. Neurocytol. 22:1060–1072
  • Gertler, F., Niebuhr, K., Reinhard, M., Wehland, J., and Soriano, P.. 1996. Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics. Cell 87:227–239
  • Gieselmann, R., Kwiatkowski, D. J., Janmey, P. A., and Witke, W.. 1995. Distinct biochemical characteristics of the two human profilin isoforms. Eur. J. Biochem. 229:621–628
  • Giesemann, T., Rathke-Hartlieb, S., Rothkegel, M., Bartsch, J. W., Buchmeier, S., Jockusch, B. M., and Jockusch, H.. 1999. A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with SMN in nuclear gems. J. Biol. Chem. 274:37908–37914
  • Goldschmidt-Clermont, P. J., Machesky, L. M., Baldassare, J. J., and Pollard, T. D.. 1990. The actin-binding protein profilin binds to PIP2 and inhibits its hydrolysis by phospholipase C. Science 247:1575–1578
  • Goldschmidt-Clermont, P. J., Kim, J. W., Machesky, L. M., Rhee, S. G., and Pollard, T. D.. 1991. Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. Science 251:1231–1233
  • Haarer, B. K., Petzold, A. S., and Brown, S. S.. 1993. Mutational analysis of yeast profilin. Mol. Cell. Biol. 13:7864–7873
  • Hogan, B., Beddington, R., Constantini, F., and Lacy, E.. 1994. Immunocytochemistry of whole mount embryos Manipulation of the mouse embryo. Hogan, B., Beddington, R., Constantini, F., and Lacy, E. 340–367 Cold Spring Harbor Laboratory Press, Plainview, N.Y
  • Honoré, B., Madsen, P., Andersen, A. H., and Leffers, H.. 1993. Cloning and expression of a novel human profilin variant, profilin II. FEBS Lett. 330:151–155
  • Jonckheere, V., Lambrechts, A., Vandekerckhove, J., and Ampe, C.. 1999. Dimerization of profilin II upon binding the (GP5)3 peptide from VASP overcomes the inhibition of actin nucleation by profilin II and thymosin β4. FEBS Lett. 447:257–263
  • Kang, F., Purich, D., and Southwick, F.. 1999. Profilin promotes barbed-end actin filament assembly without lowering the critical concentration. J. Biol. Chem. 274:36963–36972
  • Kozak, M.. 1989. The scanning model for translation: an update. J. Cell Biol. 108:229–241
  • Kwiatkowski, D. J., and Bruns, G. A.. 1988. Human profilin. Molecular cloning, sequence comparison, and chromosomal analysis. J. Biol. Chem. 263:5910–5915
  • Lambrechts, A., Van Damme, J., Goethals, M., Vandekerckhove, J., and Ampe, C.. 1995. Purification and characterization of bovine profilin II; actin, poly(L-proline) and inositolphospholipid binding. Eur. J. Biochem. 230:281–286
  • Lambrechts, A., Verschelde, J.-L., Jonckheere, V., Goethals, M., Vandekerckhove, J., and Ampe, C.. 1997. The mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences. EMBO J. 16:484–494
  • Lambrechts, A. I., Kwiatkowski A., Lanier L. M., Bear J. E., Vandeckerckhove J., Ampe C., and Gertler F. B.. PKA phosphorylation of EVL, a Mena/VASP relative, regulates its interaction with actin and SH3-domains. J. Biol. Chem., in press.
  • Lanier, L., Gates, M., Witke, W., Menzies, S., Wehman, A., Macklis, J., Kwiatkowski, D., Soriano, P., and Gertler, F.. 1999. Mena is required for neurulation and commissure formation. Neuron 22:313–325
  • Lassing, I., and Lindberg, U.. 1985. Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin. Nature 314:472–474
  • Machesky, L. M., Atkinson, S. J., Ampe, C., Vandekerckhove, J., and Pollard, T. D.. 1994. Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J. Cell Biol. 127:107–115
  • Mahoney, N. M., Janmey, P. A., and Almo, S. C.. 1997. Structure of the profilin-poly-L-proline complex involved in morphogenesis and cytoskeletal regulation. Nat. Struct. Biol. 4:953–960
  • Mahoney, N. M., Rozwarski, D. A., Fedorov, E., Fedorov, A. A., and Almo, S. C.. 1999. Profilin binds proline-rich ligands in two distinct amide backbone orientations. Nat. Struct. Biol. 6:666–671
  • Mertens, N., Remaut, E., and Fiers, W.. 1995. Tight transcriptional control mechanism ensures stable high-level expression from T7 promoter-based expression plasmids. Bio/Technology 13:175–179
  • Nodelman, I. M., Bowman, G. D., Lindberg, U., and Schutt, C. E.. 1999. X-ray structure determination of human profilin II: comparative structural analysis of human profilins. J. Mol. Biol. 294:1271–1285
  • Pantaloni, D., and Carlier, M.-F.. 1993. How profilin promotes actin filament assembly in the presence of thymosineβ4. Cell 75:1007–1014
  • Pardee, J., and Spudich, J.. 1982. Purification of muscle actin. Methods Cell Biol. 24:271–289
  • Perelroizen, E. C., Marchand, J.-B., Blanchoin, L., Didry, D., and Carlier, M.-F.. 1994. Interaction of profilin with G-actin and poly(L-proline). Biochemistry 33:8472–8478
  • Petrella, E. C., Machesky, L. M., Kaiser, D. A., and Pollard, T. D.. 1996. Structural requirements and thermodynamics of the interaction of proline peptides with profilin. Biochemistry 35:16535–16543
  • Reinhard, M., Giehl, K., Abel, K., Haffner, C., Jarchau, T., Hoppe, V., Jockush, B., and Walter, U.. 1995. The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J. 14:1583–1589
  • Schutt, C., Myslik, J., Rozycki, M., Goonesekere, N., and Lindberg, U.. 1993. The structure of crystalline profilin-beta-actin. Nature 365:810–816
  • Small, J. V., Rottner, K., Kaverina, I., and Anderson, K. I.. 1998. Assembling an actin cytoskeleton for cell attachment and movement. Biochim. Biophys. Acta 1404:271–281
  • Sohn, R. H., and Goldschmidt-Clermont, P. J.. 1994. Profilin: at the crossroads of signal transduction and the actin cytoskeleton. Bioessays 16:465–472
  • Sohn, R. H., Chen, J., Koblan, K. S., Bray, P. F., and Goldschmidt-Clermont, P. J.. 1995. Localization of a binding site for phosphatidylinositol 4,5-bisphosphate on human profilin. J. Biol. Chem. 270:21114–21120
  • Spudich, J., and Watt, S.. 1971. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. Biol. Chem. 246:4866–4871
  • Stossel, T. P., Hartwig, J. H., Janmey, P. A., and Kwiatkowski, D. J.. 1999. Cell crawling two decades after Abercrombie. Biochem. Soc. Symp. 65:267–280
  • Suetsugu, S., Miki, H., and Takenawa, T.. 1998. Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis. EMBO J. 17:6516–6526
  • Wang, X., Kibschull, M., Laue, M. M., Lichte, B., Petrasch-Parwez, E., and Kilimann, M. W.. 1999. Aczonin, a 550-kD putative scaffolding protein of presynaptic active zones, shares homology regions with Rim and Bassoon and binds profilin. J. Cell Biol. 147:151–162
  • Wasserman, S.. 1998. FH proteins as cytoskeletal organizers. Trends Cell Biol. 8:111–115
  • Watanabe, N., Madaule, P., Reid, T., Ishizaki, T., Watanabe, G., Kakizuka, A., Saito, Y., Nakao, K., Jockusch, B., and Narumiya, S.. 1997. P140mDia, a mammalian homolog of Drosophila diaphanous, is a target protein for Rho small GTPase and is a ligand for profilin. EMBO J. 16:3044–3056
  • Widada, J. S., Ferraz, C., and Liautard, J. P.. 1989. Total coding sequence of profilin cDNA from Mus musculus macrophage. Nucleic Acids Res. 17:2855
  • Witke, W., Podtelejnikov, A. V., Di Nardo, A., Sutherland, J. D., Gurniak, C. B., Dotti, C., and Mann, M.. 1998. In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly. EMBO J. 17:967–976

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