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Pediatric Hematology and Oncology Volume 31, 2014 - Issue 4
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ORIGINAL ARTICLESNon-Malignant Hematology

Quantification of Nucleated Cells, CD34-Positive Cells and CFU-GM Colonies in Single Bone Marrow Samples and Bone Marrow Harvests Derived from Healthy Children

Michael M. SchündelnDepartment of Pediatric Hematology and Oncology, University Hospital, Essen, GermanyCorrespondence[email protected]
,
Gabriele WaldeDepartment of Pediatric Hematology and Oncology, University Hospital, Essen, Germany
,
Oliver BasuDepartment of Pediatric Hematology and Oncology, University Hospital, Essen, Germany
,
Werner HaversDepartment of Pediatric Hematology and Oncology, University Hospital, Essen, Germany
&
Bernhard KremensDepartment of Pediatric Hematology and Oncology, University Hospital, Essen, Germany
Pages 340-348 | Received 08 Dec 2013, Accepted 09 Dec 2013, Published online: 05 Feb 2014

REFERENCES

  • Civin CI, Strauss LC, Brovall C, Antigenic analysis of hematopoiesis III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG 1a-cells. J Immunol. 1984;133:157–165.
  • Metcalf D. Hemopoietic Colonies. Berlin: Springer-Verlag; 1977:1–227.
  • To LB, Haylock DN, Simmons PJ, Juttner CA. The biology and clinical uses of blood stem cells. Blood. 1977;89:2233–2258.
  • Ivanovic, Z. Hematopoietic stem cells in research and clinical applications: The “CD34 issue”. World J Stem Cells. 2010;2:18–23.
  • Sutherland DR, Anderson L, Keeney M, The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematother. 1996;5:213–226.
  • Bøyum A. Isolation of human blood monocytes with Nycodenz, a new non-ionic iodinated gradient medium. Scand J Immunol. 1983;17:429–436.
  • Hawkins WW, Speck E, Leonard VG. Variation of the hemoglobin level with age and sex. Blood. 1954;9:999–1007.
  • Machida U, Tojo A, Takahashi S, The effect of granulocyte colony-stimulating factor administration in healthy donors before bone marrow harvesting. Br J Haematol. 2000;108:141–153.
  • Lannert H, Able T, Becker S, Optimizing BM harvesting from normal adult donors. Bone Marrow Transplant. 2008;42:443–447.
  • Laughlin MJ, Christiansen NP, Herzig GP, CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy. Cytometry. 1996;26:235–242.
  • Brooimans RA, Kraan J, van Putten W, Flow cytometric differential of leukocyte populations in normal bone marrow: influence of peripheral blood contamination. Cytometry B Clin Cytom. 2009;76:18–26.
  • Bender JG, Unverzagt KL, Walker DE, Identification and comparison of CD34+ cells and their subpopulations from normal peripheral blood and bone marrow using multicolor flow cytometry. Blood. 1991;77:2591–2596.
  • Syrjala M, Ruutu T, Jansson SE. A flow cytometric assay of CD34-positive cell populations in the bone marrow. Br J Haematol. 1994;88:679–684.
  • Cho SH, Chung IJ, Lee JJ, Comparison of CD34+ subsets and clonogenicity in human bone marrow, granulocyte colony-stimulating factor-mobilized peripheral blood, and cord blood. J Korean Med Sci. 1999;14:520–525.
  • Carvalho JM, Souza MK, Buccheri V, CD34-positive cells and their subpopulations characterized by flow cytometry analyses on the bone marrow of healthy allogenic donors. Sao Paulo Med J. 2009;127:12–18.
  • Isaikina Y, Kustanovich A, Svirnovski A. Growth kinetics and self-renewal of human mesenchymal stem cells derived from bone marrow of children with oncohematological diseases during expansion in vitro. Exp Oncol. 2006;28:146–151.
  • Serke S, Arseniev L, Watts M, Imprecision of counting CFU-GM colonies and CD34-expressing cells. Bone Marrow Transplant. 1997;20:57–61.
  • Rujkijyanont P, Hipps J, Gan K, Prediction of CD34(+) cell yield in hematopoietic cell products from children by peripheral blood CD34(+) cell counts. Cytotherapy. 2012;14:473–482.
  • Ince EU, Ertem M, Dogu F, et al. The impact of donor age and sex on the nucleated cell and CD34 count in healthy bone marrow donors. Poster P473 presented at the 39th Annual Meeting of the European Group for Blood and Marrow Transplantation. London, UK; 2013.
  • Rego EM, Garcia AB, Viana SR, Falcao RP. Age-related changes of lymphocyte subsets in normal bone marrow biopsies. Cytometry. 1998;34:22–29.
  • Jensen K, Schaffer L, Olstad OK, Striking decrease in the total precursor B-cell compartment during early childhood as evidenced by flow cytometry and gene expression changes. Pediatr Hematol Oncol. 2010;27:31–45.

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