1,627
Views
16
CrossRef citations to date
0
Altmetric
Report

A combinatorial mutagenesis approach for functional epitope mapping on phage-displayed target antigen

Application to antibodies against epidermal growth factor

, &
Pages 637-648 | Received 27 Jan 2014, Accepted 21 Feb 2014, Published online: 03 Mar 2014

References

  • Boyman O, Kovar M, Rubinstein MP, Surh CD, Sprent J. Selective stimulation of T cell subsets with antibody-cytokine immune complexes. Science 2006; 311:1924 - 7; http://dx.doi.org/10.1126/science.1122927; PMID: 16484453
  • Klein C, Lammens A, Schäfer W, Gorges S, Schwaiger M, Mössner E, Hopfner KP, Umaña P, Niederfellner G. Epitope interactions of monoclonal antibodies targeting CD20 and their relationships to functional properties. MAbs 2013; 5:1 - 12; PMID: 23254906
  • Rojas G, Pupo A, Leon K, Avellanet J, Carmenate T, Sidhu S. Deciphering the molecular bases of the biological effects of antibodies against Interleukin-2: a versatile platform for fine epitope mapping. Immunobiology 2013; 218:105 - 13; http://dx.doi.org/10.1016/j.imbio.2012.02.009; PMID: 22459271
  • Rojas G, Cabrera Infante Y, Pupo A, Carmenate T. Fine epitope specificity of antibodies against Interleukin-2 explains their paradoxical immunomodulatory effects. MAbs 2013; 6:273 - 85; PMID: 24253188
  • Greenspan NS, Di Cera E. Defining epitopes: It’s not as easy as it seems. Nat Biotechnol 1999; 17:936 - 7; http://dx.doi.org/10.1038/13590; PMID: 10504677
  • Davies DR, Cohen GH. Interactions of protein antigens with antibodies. Proc Natl Acad Sci U S A 1996; 93:7 - 12; http://dx.doi.org/10.1073/pnas.93.1.7; PMID: 8552677
  • Van Regenmortel MH. Structural and functional approaches to the study of protein antigenicity. Immunol Today 1989; 10:266 - 72; http://dx.doi.org/10.1016/0167-5699(89)90140-0; PMID: 2478146
  • Benjamin DC, Perdue SS. Site-directed mutagenesis in epitope mapping. Methods 1996; 9:508 - 15; http://dx.doi.org/10.1006/meth.1996.0058; PMID: 8812706
  • Frank R. The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports--principles and applications. J Immunol Methods 2002; 267:13 - 26; http://dx.doi.org/10.1016/S0022-1759(02)00137-0; PMID: 12135797
  • Di Niro R, Ferrara F, Not T, Bradbury ARM, Chirdo F, Marzari R, Sblattero D. Characterizing monoclonal antibody epitopes by filtered gene fragment phage display. Biochem J 2005; 388:889 - 94; http://dx.doi.org/10.1042/BJ20041983; PMID: 15720292
  • Felici F, Castagnoli L, Musacchio A, Jappelli R, Cesareni G. Selection of antibody ligands from a large library of oligopeptides expressed on a multivalent exposition vector. J Mol Biol 1991; 222:301 - 10; http://dx.doi.org/10.1016/0022-2836(91)90213-P; PMID: 1720463
  • Perosa F, Favoino E, Vicenti C, Guarnera A, Racanelli V, De Pinto V, Dammacco F. Two structurally different rituximab-specific CD20 mimotope peptides reveal that rituximab recognizes two different CD20-associated epitopes. J Immunol 2009; 182:416 - 23; PMID: 19109173
  • Saphire EO, Montero M, Menendez A, van Houten NE, Irving MB, Pantophlet R, Zwick MB, Parren PWHI, Burton DR, Scott JK, et al. Structure of a high-affinity “mimotope” peptide bound to HIV-1-neutralizing antibody b12 explains its inability to elicit gp120 cross-reactive antibodies. J Mol Biol 2007; 369:696 - 709; http://dx.doi.org/10.1016/j.jmb.2007.01.060; PMID: 17445828
  • Smith GP, Petrenko VA. Phage Display. Chem Rev 1997; 97:391 - 410; http://dx.doi.org/10.1021/cr960065d; PMID: 11848876
  • Kunkel TA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc Natl Acad Sci U S A 1985; 82:488 - 92; http://dx.doi.org/10.1073/pnas.82.2.488; PMID: 3881765
  • Freyre M, Vazquez J, Duarte C, Ferra E, Lopez I, Arteaga N, Alfaro A, Gavilondo J. Anticuerpos monoclonales que reconocen los factores de crecimiento epidérmico humano y murino. Interferon y Biotecnologia 1989; 6:32 - 43
  • Ogiso H, Ishitani R, Nureki O, Fukai S, Yamanaka M, Kim J-H, Saito K, Sakamoto A, Inoue M, Shirouzu M, et al. Crystal structure of the complex of human epidermal growth factor and receptor extracellular domains. Cell 2002; 110:775 - 87; http://dx.doi.org/10.1016/S0092-8674(02)00963-7; PMID: 12297050
  • Yang Z, Nielsen R, Hasegawa M. Models of amino acid substitution and applications to mitochondrial protein evolution. Mol Biol Evol 1998; 15:1600 - 11; http://dx.doi.org/10.1093/oxfordjournals.molbev.a025888; PMID: 9866196
  • Yampolsky LY, Stoltzfus A. The exchangeability of amino acids in proteins. Genetics 2005; 170:1459 - 72; http://dx.doi.org/10.1534/genetics.104.039107; PMID: 15944362
  • Jonson PH, Petersen SB. A critical view on conservative mutations. Protein Eng 2001; 14:397 - 402; http://dx.doi.org/10.1093/protein/14.6.397; PMID: 11477218
  • Mainet D, Caccamo F, Duarte C. A fast and sensitive noncompetitive streptavidin-biotin enzyme immunoassay for human epidermal growth factor. Biotecnol Apl 1993; 10:176 - 82
  • Chao G, Cochran JR, Wittrup KD. Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display. J Mol Biol 2004; 342:539 - 50; http://dx.doi.org/10.1016/j.jmb.2004.07.053; PMID: 15327953
  • Levy R, Forsyth CM, LaPorte SL, Geren IN, Smith LA, Marks JD. Fine and domain-level epitope mapping of botulinum neurotoxin type A neutralizing antibodies by yeast surface display. J Mol Biol 2007; 365:196 - 210; http://dx.doi.org/10.1016/j.jmb.2006.09.084; PMID: 17059824
  • Jespers L, Jenné S, Lasters I, Collen D. Epitope mapping by negative selection of randomized antigen libraries displayed on filamentous phage. J Mol Biol 1997; 269:704 - 18; http://dx.doi.org/10.1006/jmbi.1997.1077; PMID: 9223635
  • Morrison KL, Weiss GA. Combinatorial alanine-scanning. Curr Opin Chem Biol 2001; 5:302 - 7; http://dx.doi.org/10.1016/S1367-5931(00)00206-4; PMID: 11479122
  • Weiss GA, Watanabe CK, Zhong A, Goddard A, Sidhu SS. Rapid mapping of protein functional epitopes by combinatorial alanine scanning. Proc Natl Acad Sci U S A 2000; 97:8950 - 4; http://dx.doi.org/10.1073/pnas.160252097; PMID: 10908667
  • Pál G, Kouadio J-LK, Artis DR, Kossiakoff AA, Sidhu SS. Comprehensive and quantitative mapping of energy landscapes for protein-protein interactions by rapid combinatorial scanning. J Biol Chem 2006; 281:22378 - 85; http://dx.doi.org/10.1074/jbc.M603826200; PMID: 16762925
  • Jin L, Wells JA. Dissecting the energetics of an antibody-antigen interface by alanine shaving and molecular grafting. Protein Sci 1994; 3:2351 - 7; http://dx.doi.org/10.1002/pro.5560031219; PMID: 7538848
  • Larocca D, Jensen-Pergakes K, Burg MA, Baird A. Receptor-targeted gene delivery using multivalent phagemid particles. Mol Ther 2001; 3:476 - 84; http://dx.doi.org/10.1006/mthe.2001.0284; PMID: 11319907
  • Souriau C, Gracy J, Chiche L, Weill M. Direct selection of EGF mutants displayed on filamentous phage using cells overexpressing EGF receptor. Biol Chem 1999; 380:451 - 8; http://dx.doi.org/10.1515/BC.1999.059; PMID: 10355631
  • Stortelers C, van der Woning SP, Jacobs-Oomen S, Wingens M, van Zoelen EJJ. Selective formation of ErbB-2/ErbB-3 heterodimers depends on the ErbB-3 affinity of epidermal growth factor-like ligands. J Biol Chem 2003; 278:12055 - 63; http://dx.doi.org/10.1074/jbc.M211948200; PMID: 12556529
  • van der Woning SP, van Rotterdam W, Nabuurs SB, Venselaar H, Jacobs-Oomen S, Wingens M, Vriend G, Stortelers C, van Zoelen EJJ. Negative constraints underlie the ErbB specificity of epidermal growth factor-like ligands. J Biol Chem 2006; 281:40033 - 40; http://dx.doi.org/10.1074/jbc.M603168200; PMID: 17032651
  • Bach M, Hölig P, Schlosser E, Völkel T, Graser A, Müller R, Kontermann RE. Isolation from phage display libraries of lysine-deficient human epidermal growth factor variants for directional conjugation as targeting ligands. Protein Eng 2003; 16:1107 - 13; http://dx.doi.org/10.1093/protein/gzg145; PMID: 14983093
  • Rodríguez PC, Rodríguez G, González G, Lage A. Clinical development and perspectives of CIMAvax EGF, Cuban vaccine for non-small-cell lung cancer therapy. MEDICC Rev 2010; 12:17 - 23; PMID: 20387330
  • Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffiths AD, Winter G. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J Mol Biol 1991; 222:581 - 97; http://dx.doi.org/10.1016/0022-2836(91)90498-U; PMID: 1748994
  • Fellouse FA, Sidhu SS. Making antibodies in bacteria. In Making and using antibodies. A practical handbook. G.C. Howard, and M.R. Kaser, CRC Press, Boca Raton, Florida, 2007, pp 157-177.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.