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Review

Optimization of protein expression systems for modern drug discovery

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Pages 67-78 | Published online: 09 Jan 2014

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Fredrik Öberg & Kristina Hedfalk. (2013) Recombinant production of the human aquaporins in the yeast Pichia pastoris (Invited Review). Molecular Membrane Biology 30:1, pages 15-31.
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Jessica Lynn Grey & David H Thompson. (2010) Challenges and opportunities for new protein crystallization strategies in structure-based drug design. Expert Opinion on Drug Discovery 5:11, pages 1039-1045.
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Philipp Moritz Fricke, Angelika Klemm, Michael Bott & Tino Polen. (2021) On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases. Applied Microbiology and Biotechnology 105:9, pages 3423-3456.
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Philipp Moritz Fricke, Tobias Link, Jochem Gätgens, Christiane Sonntag, Maike Otto, Michael Bott & Tino Polen. (2020) A tunable l-arabinose-inducible expression plasmid for the acetic acid bacterium Gluconobacter oxydans. Applied Microbiology and Biotechnology 104:21, pages 9267-9282.
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Sibusiso B. Maseko, Deidre Govender, Thavendran Govender, Tricia Naicker, Johnson Lin, Glenn E. M. Maguire & Hendrik G. Kruger. (2019) Optimized Procedure for Recovering HIV-1 Protease (C-SA) from Inclusion Bodies. The Protein Journal 38:1, pages 30-36.
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Hongbo Li & Yuxian Xia. (2018) Improving the secretory expression of active recombinant AaIT in Pichia pastoris by changing the expression strain and plasmid. World Journal of Microbiology and Biotechnology 34:7.
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Amrita Singh-Blom, Randall A. Hughes & Andrew D. Ellington. (2014) An amino acid depleted cell-free protein synthesis system for the incorporation of non-canonical amino acid analogs into proteins. Journal of Biotechnology 178, pages 12-22.
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Andriy Luzhetskyy, Gabriele Weitnauer & Andreas Bechthold. 2012. Pharmaceutical Biotechnology. Pharmaceutical Biotechnology 15 41 .
Filippo Caschera, Mark A. Bedau, Andrew Buchanan, James Cawse, Davide de Lucrezia, Gianluca Gazzola, Martin M. Hanczyc & Norman H. Packard. (2011) Coping with complexity: Machine learning optimization of cell-free protein synthesis. Biotechnology and Bioengineering 108:9, pages 2218-2228.
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Feng Zhou, Job D. Cardoza, Scott B. Ficarro, Guillaume O. Adelmant, Jean-Bernard Lazaro & Jarrod A. Marto. (2010) Online Nanoflow RP−RP-MS Reveals Dynamics of Multicomponent Ku Complex in Response to DNA Damage. Journal of Proteome Research 9:12, pages 6242-6255.
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Nicklas Bonander, Richard AJ Darby, Ljuban Grgic, Nagamani Bora, Jikai Wen, Saverio Brogna, David R Poyner, Michael AA O'Neill & Roslyn M Bill. (2009) Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield. Microbial Cell Factories 8:1.
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Maria Hrmova & Geoffrey B. Fincher. 2009. Plant Genomics. Plant Genomics 199 227 .
Koji Enomoto, Ken-Ichiro Uwabe, Shoichi Naito, Jyunji Onoda, Akira Yamauchi, Yoshito Numata & Hiroshi Takemoto. (2008) A double epitope tag for quantification of recombinant protein using fluorescence resonance energy transfer. Analytical Biochemistry 380:2, pages 249-256.
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Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M. Klingeman, Xiaohu Wang, Joseph Petrosino, Lisa Hemphill, Xiufeng Wan, Adam B. Leaphart, George M. Weinstock, Timothy Palzkill & Jizhong Zhou. (2008) Generation and Validation of a Shewanella oneidensis MR-1 Clone Set for Protein Expression and Phage Display. PLoS ONE 3:8, pages e2983.
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Yuri M. Shlyapnikov, Yaroslav A. Andreev, Sergey A. Kozlov, Alexander A. Vassilevski & Eugene V. Grishin. (2008) Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom. Protein Expression and Purification 60:1, pages 89-95.
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Felix Freuler, Thomas Stettler, Marco Meyerhofer, Lukas Leder & Lorenz M. Mayr. (2008) Development of a novel Gateway-based vector system for efficient, multiparallel protein expression in Escherichia coli. Protein Expression and Purification 59:2, pages 232-241.
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Ai-Sheng Xiong, Ri-He Peng, Jing Zhuang, Feng Gao, Yi Li, Zong-Ming Cheng & Quan-Hong Yao. (2008) Chemical gene synthesis: strategies, softwares, error corrections, and applications. FEMS Microbiology Reviews 32:3, pages 522-540.
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H. Jhoti. 2008. Sparking Signals. Sparking Signals 169 185 .
Harren Jhoti, Anne Cleasby, Marcel Verdonk & Glyn Williams. (2007) Fragment-based screening using X-ray crystallography and NMR spectroscopy. Current Opinion in Chemical Biology 11:5, pages 485-493.
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