Chemical Composition and Antimicrobial Activity of Essential Oil of Matricaria recutita

Figures & data

TABLE 1 Chemical composition of essential oil of Matricaria recutita

Peak number	Components	Chamomile %	Identification methods
1	α-Pinene	8	MS,RI
2	Camphene	9.11	MS,RI
3	Sabinene	4.87	MS,RI
4	D-3-Carene	0.1	MS,RI
5	α-Terpinene	2.32	MS,RI
6	P-Cymene	0.5	MS,RI
7	β-Phellandrene	0.9	MS,RI
8	Limonene	6	MS,RI
9	1,8-Cineole	7.14	MS,RI
10	Benzeneacetaldehyde	0.12	MS,RI
11	γ-Terpinene	0.9	MS,RI
12	Artemisiaketone	0.23	MS,RI
13	Z-Sabinenehydrate	0.98	MS,RI
14	α-Linalool	0.12	MS,RI
15	α-Thujone	0.64	MS,RI
16	β-Thujone	0.89	MS,RI,Co
17	E-Sabinol	0.87	MS,RI,Co
18	Camphor	6.54	MS,RI
19	Borneol	0.45	MS,RI
20	4-Terpineol	0.6	MS,RI
21	α-Terpineol	0.61	MS,RI,Co
22	E-Piperitol	0.54	MS,RI,Co
23	α-Cubebene	0.2	MS,RI
24	α-Terpinylacetate	0.7	MS,RI
25	α-Isocomene	0.15	MS,RI
26	β-Elemene	0.78	MS,RI
27	α-Funebrene	0.31	MS,RI
28	Isocaryophyllene	0.3	MS,RI,Co
29	β-Caryophyllene	0.5	MS,RI
30	E-β-farnesene	0.43	MS,RI,Co
31	GermacreneD	0.7	MS,RI
32	Bicyclogermacrene	0.23	MS,RI
33	E-Nerolidol	0.8	MS,RI
34	Spathulenol	0.34	MS,RI
35	Caryophyllene oxide	0.23	MS,RI
36	α-Bisabolol oxide	38	MS,RI
37	α-Bisabolol	1.11	MS,RI,Co
38	Chamazulene	1.31	MS,RI,Co
39	α -Farnesene	1	MS,RI,Co
40	β -Farnesene	1.54	MS,RI,Co
	Yield	0.82
	Total	80.76

TABLE 2 Antibacterial activity of essential oils (1.0 μg/mL) in disc-diffusion method, inhibition zones in mm

Microorganisms	Chamomile %	Streptomycin
Gram positive
Staphylococcus aureus	30	22
Bacillus cereus	36	22
Bacillus subtilis	32	22
Gram negative
Shigella shiga	25	17
Shigella sonnei	19	18
Pseudomonua aeruginosa	19	18
Proteus sp.	16	15

Diameter of inhibition zones (mm) including the diameter of disc (6 mm).

TABLE 3 Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of essential oil from Matricaria chamomilla

	Essential oil chamomile		Streptomycin
Microorganisms	MIC	MBC	MIC	MBC
Gram positive
Staphylococcus aureus	0.011	0.5	0.13	1
Bacillus cereus	0.022	1.5	0.12	1.5
Bacillus subtilis	0.03	1.5	0.115	1
Gram negative
Shigella shiga	0.14	3	0.11	5
Shigella sonnei	0.2	3	0.1	3
Pseudomonua aeruginosa	4	8	5	8
Proteus sp.	0.15	3	0.1	4

MIC: minimum inhibitory concentration (values in μg/ml); MBC: minimum bactericidal concentration (values in μg/ml); All tests were done in duplicate; three replications were done for oil.

TABLE 4 Antibacterial activity of essential oils components (1.0 μg/ml) in disc-diffusion method, inhibition zones in mm

Microorganisms	α-Pinene	Camphene	Sabinene	Limonene	1,8-Cineole	Camphor	Bisabolol	Bisabolol oxide	Streptomycin
Gram positive
Staphylococcus aureus	18	16	14	10	15	20	15	18	15
Bacillus cereus	20	25	20	12	20	22	20	35	20
Bacillus subtilis	20	25	18	12	15	22	15	22	20
Gram negative
Shigella shiga	12	13	10	5	16	5	15	20	15
Shigella sonnei	14	15	10	5	12	5	15	20	10
Pseudomonua aeruginosa	18	5	10	4	10	15	12	12	10
Proteus sp.	15	15	10	5	10	10	15	20	10

Diameter of inhibition zones (mm) including the diameter of disc (6 mm).

TABLE 5 Antibacterial activity of essential oils components (MIC and MBC—μg/ml), microdilution method

	α-Pinene		Camphene		Sabinene		Limonene		1,8-Cineole		Camphor		Bisabolol		Bisabolol oxide		Streptomycin
Microorganisms	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC	MIC	MBC
Gram positive
Staphylococcus aureus	5	6	5	5	5	6	5	4	6	5	5	5	1.5	1.5	0.5	0.5	8	7
Bacillus cereus	5	4	5	5	5	5	6	4	5	5	5	4	1.5	1.5	0.5	0.5	6	5
Bacillus subtilis	5	6	5	5	4	4	5	4	5	5	5	4	1	1	0.5	0.5	8	5
Gram negative
Shigella shiga	6	6	5	10	6	6	7	8	5	5	10	10	4	4	1	1.5	10	9
Shigella sonnei	7	6	6	10	5	5	7	4	5	6	8	4	4	4	3	1.5	5	9
Pseudomonua aeruginosa	12	12	10	10	8	8	10	12	7	10	5	7	6	4	3	1.5	12	9
Proteus sp.	8	6	10	10	8	8	7	10	10	8	7	8	4	3	3	1.5	5	89

MIC: minimum inhibitory concentration (values in μg/ml); MBC: minimum bactericidal concentration (values in μg/ml);

All tests were done in duplicate; three replications were done for component.