Determination of the Total Antioxidant Capacity and Quantification of Phenolic Compounds of Different Solvent Extracts of Black Mustard Seeds (Brassica nigra)

Figures & data

TABLE 1 Yield of the dried extract (g) as a percentage weight of the starting fresh Brassica nigra seed powder

Solvent	Extraction yield (g)	Percentage yield (%)
DCM	3.68 ± 0.12	20.8
EtOAc	2.59 ± 0.13	13.7
EtOH	4.89 ± 0.19	25.4
HEX	2.17 ± 0.10	11.7
MeOH	3.66 ± 0.24	19.2
Water	5.24 ± 0.22	27.8

FIGURE 1 (A) Total phenolics contents; (B) ORAC; (C) DPPH EC₅₀; (D) FRAP; and (E) ABTS radical scavenging activities of DCM, EtOH, EtOAc, HEX, MeOH, and water extracts of Brassica nigra. Results are expressed as mean ± SEM per gram fresh weight. *p < 0.05 versus the antioxidant values of BHT.

TABLE 2 Phenolic compounds present in the Brassica nigra extracts^a

Solvent	Catechin	Epicatechin	Gallic acid	Myricetin	Quercetin	Rutin
DCM	8.6	5.9	5.2	5.1	4.8	4.1
EtOAc	14.9	11.1	9.8	10.8	22.1	8.9
EtOH	24.7	25.9	15.8	14.9	22.3	19.5
HEX	4.4	4.1	4.3	3.8	3.9	3.5
MeOH	6.6	5.9	10.4	6.8	7.1	7.9
Water	62.9	22.8	16.4	29.8	32.9	16.1
Lower detection limit	0.03	0.03	0.05	0.04	0.03	0.08
Lower quantification limit	0.06	0.06	0.10	0.10	0.06	0.08

^aData expressed as micrograms per gram fresh weight of powder (µg/g) and presented as mean.

FIGURE 2 Representative HPLC chromatogram of the water extract where the peaks were identified as follows: 1: Catechin; 2: Quercetin; 3: Myricetin; 4: Epicatechin; 5: Rutin; 6: Gallic acid.