Amaranth Sprouts: A Potential Health Promoting and Nutritive Natural Food

Figures & data

TABLE 1 Amaranth sprouts and seeds composition

	Sprouts		Seeds
% w/w	Dry basis	Wet basis	Dry basis	Wet basis
Moisture	nd	70.69 ± 1.28		8.1 ± 2.9
Ashes	3,5	1.04 ± 0.01	3.59	3.3 ± 0.6
Protein	16	4.68 ± 0.18	16	15 ± 2
Fiber	17	4.89 ± 0.41	nd	nd
Lipids	nd	nd	6,26	5.75 ± 2.9
Carbohydrates	nd	nd	69	63 ± 7

Values are expressed as mean of duplicate samples ± standard deviation; nd: not determined.

FIGURE 1 Tricine-SDS-PAGE. I: Seed isolate; SI: sprout isolate; SS: sprout supernatant; M: standard proteins; MW: standard molecular masses.

FIGURE 2 Gel filtration chromatography. I: Seed isolate; SI: sprout isolate; SS: sprout supernatant. Molecular markers elution volumes are indicated with arrows (29 kDa and 6.5 kDa). Percentages under the chromatograms correspond to each profile peaks area between the A and B lines.

TABLE 2 Amaranth sprouts in vitro biological activity

	IC50 (mg/mL)		TEAC (µmol/mg protein)		ORAC (mg Trolox/mg protein)
	NDg	Dg	NDg	Dg	NDg	DG
I	0.67 ± 0.18^a	0.24 ± 0.10^b	0.26 ± 0.05^c	0.75 ± 0.20^d	0.12 ± 0.03^f	0.11 ± 0.05^f
SI	0.92 ± 0.60^a	0.26 ± 0.07^b	0.32 ± 0.05^c	0.72 ± 0.08^d	0.11 ± 0.03^f	0.11 ± 0.08^f
SS	0.78 ± 0.16^a	0.18± 0.03^b	0.53 ± 0.03^e	1.00 ± 0.18^e	0.45 ± 0.10^g	0.19 ± 0.06^h

IC₅₀ values are expressed as mean ± standard deviation of five determinations;

TEAC and ORAC values, as mean ± standard deviation of three determinations;

Different superscript letters in rows and/or columns means significant differences (p < 0.05);

NDg: non-digested samples; Dg: digested samples.

TABLE 3 ABTS⁺ protein and extract scavenging capacity*

	% Scavenger capacity
	Protein	Hydrophobic extract*
I	55.5 ± 10^a	10.5 ± 1.7^b
SI	44.3 ± 7^a	10.0 ± 0.88^b
SS	73.1 ± 14^c	46.0 ± 5.3^a

*Hydrophobic extract equivalent to 5 mg/mL of the corresponding protein sample;

Values of scavenging capacity are expressed as duplicate sample mean ± standard deviation;

Different superscript letters in rows and/or columns means significant differences (p < 0.05).