Figures & data
TABLE 1 Purification of polyphenol oxidase (PPO) from Hemşin apple (Malus communis L.)
FIGURE 1 DE52-Cellulose (Diethylaminoethyl cellulose) column. (-●-) symbolize the protein content, which was spectrophotometrically measured by the absorbance at 280 nm. (-○-) symbolize the PPO activity, which was measured by the activity assay. (-∆-) symbolize the linear gradient buffer which containing (0,1–1 M) NaCl.
![FIGURE 1 DE52-Cellulose (Diethylaminoethyl cellulose) column. (-●-) symbolize the protein content, which was spectrophotometrically measured by the absorbance at 280 nm. (-○-) symbolize the PPO activity, which was measured by the activity assay. (-∆-) symbolize the linear gradient buffer which containing (0,1–1 M) NaCl.](/cms/asset/54988e4d-56eb-4375-a51f-5d8c6beb9d8a/ljfp_a_1012725_f0001_b.gif)
TABLE 2 The optimum conditions for and the kinetic and thermodynamic characteristics of purified Hemşin apple (Malus communis L.) PPO activity. (kcat: turnover number, Vmax: maximum velocity, Km: Michaelis-Menten constant, Vo: catalytic efficacy, Ea: activation energy, ∆H: enthalpy)
FIGURE 2 Optimum temperature of PPO from Malus communis L. The Arrhenius plots for heat inactivation of the purified PPO.
![FIGURE 2 Optimum temperature of PPO from Malus communis L. The Arrhenius plots for heat inactivation of the purified PPO.](/cms/asset/4e17f183-ec5b-4286-9305-e349a6ebc656/ljfp_a_1012725_f0002_b.gif)
TABLE 3 The inhibition effects of metal ions on Hemşin apple (Malus communis L.) PPO activity
TABLE 4 The activation effects of metal ions on Hemşin apple (Malus communis L.) PPO activity