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Original Article

Protonography, a new technique for the analysis of carbonic anhydrase activity

, , &
Pages 277-282 | Received 28 Mar 2014, Accepted 17 Apr 2014, Published online: 09 Jul 2014

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Read on this site (22)

Giulia Nannini, Viviana De Luca, Chiara D’Ambrosio, Andrea Scaloni, Antonio Taddei, Maria Novella Ringressi, Fabio Cianchi, Fabio Staderini, Clemente Capasso, Amedeo Amedei & Claudiu T. Supuran. (2022) A comparative study of carbonic anhydrase activity in lymphocytes from colorectal cancer tissues and adjacent healthy counterparts. Journal of Enzyme Inhibition and Medicinal Chemistry 37:1, pages 1651-1655.
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Viviana De Luca, Andrea Angeli, Valeria Mazzone, Claudia Adelfio, Vincenzo Carginale, Andrea Scaloni, Fabrizio Carta, Silvia Selleri, Claudiu T. Supuran & Clemente Capasso. (2022) Heterologous expression and biochemical characterisation of the recombinant β-carbonic anhydrase (MpaCA) from the warm-blooded vertebrate pathogen malassezia pachydermatis. Journal of Enzyme Inhibition and Medicinal Chemistry 37:1, pages 62-68.
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Alessio Nocentini, Claudiu T. Supuran & Clemente Capasso. (2021) An overview on the recently discovered iota-carbonic anhydrases. Journal of Enzyme Inhibition and Medicinal Chemistry 36:1, pages 1988-1995.
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Andrea Petreni, Viviana De Luca, Andrea Scaloni, Alessio Nocentini, Clemente Capasso & Claudiu T. Supuran. (2021) Anion inhibition studies of the Zn(II)-bound ι-carbonic anhydrase from the Gram-negative bacterium Burkholderia territorii. Journal of Enzyme Inhibition and Medicinal Chemistry 36:1, pages 372-376.
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Sonia Del Prete, Silvia Bua, Claudiu T. Supuran & Clemente Capasso. (2020) Escherichia coli γ-carbonic anhydrase: characterisation and effects of simple aromatic/heterocyclic sulphonamide inhibitors. Journal of Enzyme Inhibition and Medicinal Chemistry 35:1, pages 1545-1554.
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Sonia Del Prete, Alessio Nocentini, Claudiu T. Supuran & Clemente Capasso. (2020) Bacterial ι-carbonic anhydrase: a new active class of carbonic anhydrase identified in the genome of the Gram-negative bacterium Burkholderia territorii. Journal of Enzyme Inhibition and Medicinal Chemistry 35:1, pages 1060-1068.
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Giovanna Salbitani, Sonia Del Prete, Francesco Bolinesi, Olga Mangoni, Viviana De Luca, Vincenzo Carginale, William A. Donald, Claudiu T. Supuran, Simona Carfagna & Clemente Capasso. (2020) Use of an immobilised thermostable α-CA (SspCA) for enhancing the metabolic efficiency of the freshwater green microalga Chlorella sorokiniana. Journal of Enzyme Inhibition and Medicinal Chemistry 35:1, pages 913-920.
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Rosaria Gitto, Laura De Luca, Francesca Mancuso, Sonia Del Prete, Daniela Vullo, Claudiu T. Supuran & Clemente Capasso. (2019) Seeking new approach for therapeutic treatment of cholera disease via inhibition of bacterial carbonic anhydrases: experimental and theoretical studies for sixteen benzenesulfonamide derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry 34:1, pages 1186-1192.
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Maurizio Ronci, Sonia Del Prete, Valentina Puca, Simone Carradori, Vincenzo Carginale, Raffaella Muraro, Gabriella Mincione, Antonio Aceto, Francesca Sisto, Claudiu T. Supuran, Rossella Grande & Clemente Capasso. (2019) Identification and characterization of the α-CA in the outer membrane vesicles produced by Helicobacter pylori. Journal of Enzyme Inhibition and Medicinal Chemistry 34:1, pages 189-195.
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Daniela Vullo, Sonia Del Prete, Sameh M. Osman, Fatmah A. S. Alasmary, Zeid AlOthman, William A. Donald, Clemente Capasso & Claudiu T. Supuran. (2018) Comparison of the amine/amino acid activation profiles of the β- and γ-carbonic anhydrases from the pathogenic bacterium Burkholderia pseudomallei. Journal of Enzyme Inhibition and Medicinal Chemistry 33:1, pages 25-30.
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Sonia Del Prete, Rosa Perfetto, Mosè Rossi, Fatmah A. S. Alasmary, Sameh M. Osman, Zeid AlOthman, Claudiu T. Supuran & Clemente Capasso. (2017) A one-step procedure for immobilising the thermostable carbonic anhydrase (SspCA) on the surface membrane of Escherichia coli. Journal of Enzyme Inhibition and Medicinal Chemistry 32:1, pages 1120-1128.
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Alessio Nocentini, Daniela Vullo, Sonia Del Prete, Sameh M. Osman, Fatmah A.S. Alasmary, Zeid AlOthman, Clemente Capasso, Fabrizio Carta, Paola Gratteri & Claudiu T. Supuran. (2017) Inhibition of the β-carbonic anhydrase from the dandruff-producing fungus Malassezia globosa with monothiocarbamates. Journal of Enzyme Inhibition and Medicinal Chemistry 32:1, pages 1064-1070.
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Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Vincenzo Carginale, Giovanni Sansone, Carmela M. A. Barone, Mosè Rossi, Fatmah A. S. Alasmary, Sameh M. Osman, Zeid AlOthman, Claudiu T. Supuran & Clemente Capasso. (2017) Cloning, expression and purification of the α-carbonic anhydrase from the mantle of the Mediterranean mussel, Mytilus galloprovincialis. Journal of Enzyme Inhibition and Medicinal Chemistry 32:1, pages 1029-1035.
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Rosa Perfetto, Sonia Del Prete, Daniela Vullo, Giovanni Sansone, Carmela Barone, Mosè Rossi, Claudiu T. Supuran & Clemente Capasso. (2017) Biochemical characterization of the native α-carbonic anhydrase purified from the mantle of the Mediterranean mussel, Mytilus galloprovincialis. Journal of Enzyme Inhibition and Medicinal Chemistry 32:1, pages 632-639.
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Sonia Del Prete, Viviana De Luca, Giuseppina De Simone, Claudiu T. Supuran & Clemente Capasso. (2016) Cloning, expression and purification of the complete domain of the η-carbonic anhydrase from Plasmodium falciparum. Journal of Enzyme Inhibition and Medicinal Chemistry 31:sup4, pages 54-59.
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F. Zehra Küçükbay, Hasan Küçükbay, Muhammet Tanc & Claudiu T. Supuran. (2016) Synthesis and carbonic anhydrase I, II, IV and XII inhibitory properties of N-protected amino acid – sulfonamide conjugates. Journal of Enzyme Inhibition and Medicinal Chemistry 31:6, pages 1476-1483.
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Sonia Del Prete, Viviana De Luca, Daniela Vullo, Sameh M. Osman, Zeid AlOthman, Vincenzo Carginale, Claudiu T. Supuran & Clemente Capasso. (2016) A new procedure for the cloning, expression and purification of the β-carbonic anhydrase from the pathogenic yeast Malassezia globosa, an anti-dandruff drug target. Journal of Enzyme Inhibition and Medicinal Chemistry 31:6, pages 1156-1161.
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Ayşenur Eminoğlu, Daniela Vullo, Aycan Aşık, Dilşat Nigar Çolak, Claudiu T. Supuran, Sabriye Çanakçı & Ali Osman Beldüz. (2016) Cloning, expression and biochemical characterization of a β-carbonic anhydrase from the soil bacterium Enterobacter sp. B13. Journal of Enzyme Inhibition and Medicinal Chemistry 31:6, pages 1111-1118.
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Claudiu T. Supuran & Clemente Capasso. (2016) New light on bacterial carbonic anhydrases phylogeny based on the analysis of signal peptide sequences. Journal of Enzyme Inhibition and Medicinal Chemistry 31:6, pages 1254-1260.
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Viviana De Luca, Sonia Del Prete, Daniela Vullo, Vincenzo Carginale, Pietro Di Fonzo, Sameh M Osman, Zeid AlOthman, Claudiu T Supuran & Clemente Capasso. (2016) Expression and characterization of a recombinant psychrophilic γ-carbonic anhydrase (NcoCA) identified in the genome of the Antarctic cyanobacteria belonging to the genus Nostoc. Journal of Enzyme Inhibition and Medicinal Chemistry 31:5, pages 810-817.
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Clemente Capasso & Claudiu T Supuran. (2015) Bacterial, fungal and protozoan carbonic anhydrases as drug targets. Expert Opinion on Therapeutic Targets 19:12, pages 1689-1704.
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Viviana De Luca, Sonia Del Prete, Vincenzo Carginale, Daniela Vullo, Claudiu T. Supuran & Clemente Capasso. (2015) A failed tentative to design a super carbonic anhydrase having the biochemical properties of the most thermostable CA (SspCA) and the fastest (SazCA) enzymes. Journal of Enzyme Inhibition and Medicinal Chemistry 30:6, pages 989-994.
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Sonia Del Prete, Viviana De Luca, Elena Iandolo, Claudiu T. Supuran & Clemente Capasso. (2015) Protonography, a powerful tool for analyzing the activity and the oligomeric state of the γ-carbonic anhydrase identified in the genome of Porphyromonas gingivalis. Bioorganic & Medicinal Chemistry 23:13, pages 3747-3750.
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Daniela Vullo, Viviana De Luca, Sonia Del Prete, Vincenzo Carginale, Andrea Scozzafava, Clemente Capasso & Claudiu T. Supuran. (2015) Sulfonamide inhibition studies of the γ-carbonic anhydrase from the Antarctic cyanobacterium Nostoc commune. Bioorganic & Medicinal Chemistry 23:8, pages 1728-1734.
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Özlen Güzel-Akdemir, Atilla Akdemir, Nilgün Karalı & Claudiu T. Supuran. (2015) Discovery of novel isatin-based sulfonamides with potent and selective inhibition of the tumor-associated carbonic anhydrase isoforms IX and XII. Organic & Biomolecular Chemistry 13:23, pages 6493-6499.
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